HIV-1 Nef Induces Hck/Lyn-Dependent Expansion of Myeloid-Derived Suppressor Cells Associated with Elevated Interleukin-17/G-CSF Levels.

Journal of Virology
Elena PriceputuPaul Jolicoeur

Abstract

Human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV) infection causes myelodysplasia, anemia, and accumulation of inflammatory monocytes (CD14+ CD16+) through largely unknown cellular and molecular pathways. The mouse cells thought to be equivalent to human CD14+ CD16+ cells are CD11b+ Gr1+ myeloid-derived suppressor cells (MDSC). We used HIV transgenic (Tg) mouse models to study MDSC, namely, CD4C/Nef Tg mice expressing nef in dendritic cells (DC), pDC, CD4+ T, and other mature and immature myeloid cells and CD11c/Nef Tg mice with a more restricted expression, mainly in DC and pDC. Both Tg strains showed expansion of granulocytic and CD11b+ Gr1low/int cells with MDSC characteristics. Fetal liver cell transplantation revealed that this expansion was stroma-independent and abrogated in mixed Tg/non-Tg 50% chimera. Tg bone marrow (BM) erythroid progenitors were decreased and myeloid precursors increased, suggesting an aberrant differentiation likely driving CD11b+ Gr1+ cell expansion, apparently cell autonomously in CD4C/Nef Tg mice and likely through a bystander effect in CD11c/Nef Tg mice. Hck was activated in Tg spleen, and Nef-mediated CD11b+ Gr1+ cell expansion was abrogated in Hck/Lyn-deficient Nef Tg m...Continue Reading

References

Jan 1, 1991·American Journal of Clinical Pathology·D S Karcher, A R Frost
Jan 1, 1989·Leukemia Research·Y Lunardi-IskandarC Jasmin
Aug 1, 1987·The Journal of Clinical Investigation·C C StellaD Hoelzer
Mar 1, 1987·British Journal of Haematology·M TreacyA Clark
Jun 1, 1987·British Journal of Haematology·L I ZonJ E Groopman
Dec 1, 1986·The American Journal of the Medical Sciences·C M Shenoy, J H Lin
Aug 1, 1984·The American Journal of Medicine·J L SpivakT C Quinn
Dec 1, 1995·European Journal of Immunology·N ThieblemontN Haeffner-Cavaillon
Aug 1, 1996·Journal of Pediatric Hematology/oncology·B U MuellerP A Pizzo
Mar 16, 2000·The EMBO Journal·S O ArndtH Kropshofer
Nov 2, 2002·The EMBO Journal·Agata KlejmanTomasz Skorski
Jul 20, 2006·The Journal of Biological Chemistry·Ronald P TribleThomas E Smithgall
Feb 5, 2008·The Journal of Clinical Investigation·Wenbin XiaoToshiaki Kawakami
Jul 16, 2008·Cell Host & Microbe·Simon SwinglerMario Stevenson
Sep 13, 2008·Annual Review of Microbiology·Carol A Carter, Lorna S Ehrlich
Feb 7, 2009·Nature Reviews. Immunology·Dmitry I Gabrilovich, Srinivas Nagaraj
Feb 25, 2009·The Journal of Experimental Medicine·Caspar OhnmachtDavid Voehringer
Mar 17, 2009·Science·Kang LiuMichel Nussenzweig
Oct 22, 2009·Journal of Leukocyte Biology·Woong-Ki KimKenneth Williams
Dec 19, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laure Campillo-GimenezJérôme Estaquier
Feb 2, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Donggou HeHui Xu

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Methods Mentioned

BETA
reverse transcription-PCR
FACS
flow cytometry
enzyme-linked immunosorbent assay
ELISA
fluorescence-activated cell sorter

Software Mentioned

Summit
FlowJo

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