PMID: 1702301Sep 1, 1990Paper

HIV-1 neutralizing monoclonal antibodies induced by a synthetic peptide

AIDS Research and Human Retroviruses
P J DurdaK J Weinhold

Abstract

We have developed a series of murine monoclonal antibodies to a region of the 120 kD envelope glycoprotein (gp120) of human immunodeficiency virus type 1 (HIV-1). This region has previously been implicated as a site for virus neutralization by antisera raised to recombinant proteins and by antibodies made to full-length gp120 purified from virus. The antigen employed was a synthetic peptide containing 15 amino acids, representing amino acid residues 308-322, RIQRGPGRAFVTIGK, of env gp120 (HTLV-IIIB isolate). Five of the monoclonal antibodies raised to this antigen have reactivity with gp120 from divergent strains of HIV-1 in Western blot assays. The two of these five which were tested with live cells infected with the divergent HIV-1 isolates IIIB, MN, and RF were specifically reactive by fluorescence analyses with cells infected with the MN and IIIB isolates. Four of the five monoclonal antibodies blocked the fusion of IIIB-infected cells with uninfected MOLT-4 target cells. The monoclonal antibody most reactive with MN-infected cells by fluorescence, #5025A, blocked the fusion of MN-infected cells with uninfected MOLT-4 cells. Four of the five monoclonal antibodies neutralized the IIIB isolate of HIV-1 in vitro, but none neut...Continue Reading

References

Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T J MatthewsD P Bolognesi
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T J PalkerB F Haynes
Feb 1, 1988·AIDS·E K ThomasR A Weiss
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R RuscheP J Fischinger
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J GoudsmitD C Gajdusek
Jun 1, 1988·AIDS Research and Human Retroviruses·M A SkinnerT J Matthews
Jan 24, 1985·Nature·L RatnerK Baumeister
Oct 1, 1988·AIDS Research and Human Retroviruses·P J DurdaF Wong-Staal
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W G RobeyP J Fischinger
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T J MatthewsD P Bolognesi
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J R RuscheD P Bolognesi
Jan 1, 1980·Methods in Enzymology·M Reichlin

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Citations

Jun 14, 1995·Journal of Immunological Methods·T C VanCottD L Birx
Aug 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·M FerrerM R van Schravendijk
Jun 18, 2002·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·G J GramS Olofsson
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T OhnoD W Kufe
Jul 1, 1995·The Journal of Experimental Medicine·Q J Sattentau, J P Moore
Mar 1, 1994·AIDS Research and Human Retroviruses·A Douvas, Y Takehana
Sep 1, 1991·Scandinavian Journal of Immunology·L R HaaheimA R Coates
Sep 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·H MoorjaniR T Steigbigel
Sep 8, 1998·Clinical and Diagnostic Laboratory Immunology·E M Rakowicz-SzulczynskaM Smith

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