PMID: 9443667Jan 27, 1998Paper

HIV-1 protease does not play a critical role in the early stages of HIV-1 infection

Antiviral Research
H UchidaHiroaki Mitsuya

Abstract

We asked whether human immunodeficiency virus type 1 (HIV-1) protease plays a major role in the early stages of infection (i.e. from viral entry to reverse transcription) by using various protease inhibitors (saquinavir, ritonavir, and KNI-272). When assessed in the two-day multinuclear activation of a galactosidase indicator (MAGI) assay, involving a single cycle of HIV-1 replication, all protease inhibitors failed to block infection of HeLa-CD4-LTR-beta-gal cells by HIV-1, while reverse transcriptase (RT) inhibitors (AZT and ddI) completely blocked the infection. Moreover, when HIV-1 proviral DNA synthesis was examined by polymerase chain reaction in HeLa-CD4-LTR-beta-gal cells exposed to HIV-1 and cultured in the presence of protease inhibitors, a significant amount of proviral DNA was detected, while no proviral DNA synthesis was detected when the cells were cultured in the presence of RT inhibitors. Protease inhibitors also failed to block chloramphenicol acetyltransferase (CAT) expression in HLCD4-CAT cells exposed to HIV-1, while RT inhibitors completely suppressed CAT expression. These results strongly suggest, contrary to a previous report by Nagy et al. (1994), that HIV-1 protease does not play a major role in the ear...Continue Reading

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Citations

Feb 15, 2001·Journal of Medical Virology·T GotoK Sano
Feb 13, 2009·The Tohoku Journal of Experimental Medicine·Andros TheoToshio Hattori
Oct 21, 2003·Nucleosides, Nucleotides & Nucleic Acids·Satoru KohgoHiroshi Ohrui
Jun 18, 2004·Nucleosides, Nucleotides & Nucleic Acids·Satoru KohgoHiroshi Ohrui
Dec 17, 2014·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Melanie R NicolAngela D M Kashuba
Jan 8, 2014·Reviews in Medical Virology·Vidya SagarMadhavan Nair
Oct 17, 2001·The Journal of Biological Chemistry·A GruberF Wong-Staal

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