HIV-1 viral load measurement in venous blood and fingerprick blood using Abbott RealTime HIV-1 DBS assay

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Ning TangCarole L Wallis

Abstract

HIV RNA suppression is a key indicator for monitoring success of antiretroviral therapy. From a logistical perspective, viral load (VL) testing using Dried Blood Spots (DBS) is a promising alternative to plasma based VL testing in resource-limited settings. To evaluate the analytical and clinical performance of the Abbott RealTime HIV-1 assay using a fully automated one-spot DBS sample protocol. Limit of detection (LOD), linearity, lower limit of quantitation (LLQ), upper limit of quantitation (ULQ), and precision were determined using serial dilutions of HIV-1 Virology Quality Assurance stock (VQA Rush University), or HIV-1-containing armored RNA, made in venous blood. To evaluate correlation, bias, and agreement, 497 HIV-1 positive adult clinical samples were collected from Ivory Coast, Uganda and South Africa. For each HIV-1 participant, DBS-fingerprick, DBS-venous and plasma sample results were compared. Correlation and bias values were obtained. The sensitivity and specificity were analyzed at a threshold of 1000 HIV-1 copies/mL generated using the standard plasma protocol. The Abbott HIV-1 DBS protocol had an LOD of 839 copies/mL, a linear range from 500 to 1×107copies/mL, an LLQ of 839 copies/mL, a ULQ of 1×107copies/mL,...Continue Reading

Citations

Jul 20, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Brooke E NicholsWendy Stevens
Jun 13, 2020·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Seth C KalichmanAngela M Caliendo
Aug 1, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stephanie HackettElaine J Abrams
Oct 17, 2020·Avian Pathology : Journal of the W.V.P.A·Suwarak WannaratanaSomsak Pakpinyo
May 16, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Lucia HansSergio Carmona
Jul 17, 2021·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Seth C KalichmanLisa A Eaton

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