HIV integration site selection: targeting in macrophages and the effects of different routes of viral entry.

Molecular Therapy : the Journal of the American Society of Gene Therapy
Stephen D BarrF Bushman

Abstract

We have studied the selection of HIV DNA integration sites in primary macrophages to investigate two questions. First, mature macrophages do not divide, allowing us to investigate whether HIV integration targeting differs between dividing cells and nondividing cells. We sequenced and analyzed 754 unique integration sites and found that integration in macrophages is favored in active transcription units (TUs), as was observed previously for other cell types. However, HIV integration in genes was slightly less favored in macrophages than in dividing PBMC or T cell lines. Second, we compared integration targeting by HIV-vector particles bearing either of two different envelope proteins (HIV R5 Env or VSV-G) to determine whether the mechanism of entry influenced subsequent integration targeting. Integration sites generated by HIV R5- or VSV-G-bearing particles showed no significant differences in their distributions in the human genome. Analysis of additional published integration site sequences also indicated that the route of entry did not affect integration site selection for other viral envelopes as well.

References

Jul 10, 1998·Cell·J D Boeke, S E Devine
Dec 23, 1999·Current Opinion in Genetics & Development·A F Smit
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Aug 31, 2002·Cell·Astrid R W SchröderFrederic Bushman
Jun 11, 2003·The Journal of Biological Chemistry·Goedele MaertensYves Engelborghs
Apr 30, 2004·The New England Journal of Medicine·Justin StebbingDaniel C Douek
Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Fanny TurlureAlan Engelman
Oct 14, 2004·Journal of Virology·Anna NarezkinaRichard A Katz
Oct 19, 2004·Molecular Immunology·Alessia VeraniGianfranco Pancino
Apr 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alexander G Holman, John M Coffin
May 28, 2005·The EMBO Journal·Natalia SharovaMario Stevenson
Sep 22, 2005·Nature Reviews. Microbiology·Frederic BushmanChristian Hoffmann
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Peter CherepanovAlan Engelman
Nov 29, 2005·Nature Medicine·Angela CiuffiFrederic Bushman

❮ Previous
Next ❯

Citations

Apr 25, 2014·Viruses·Amit KumarGeorges Herbein
Dec 15, 2006·PLoS Computational Biology·Charles BerryFrederic D Bushman
Dec 20, 2007·PloS One·Heather M MarshallFrederic D Bushman
Apr 16, 2008·Nucleic Acids Research·Gary P WangFrederic D Bushman
Aug 9, 2008·The Journal of Clinical Investigation·Salima Hacein-Bey-AbinaMarina Cavazzana-Calvo
Feb 26, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nicklas H StaunstrupJacob Giehm Mikkelsen
Mar 5, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gary P WangFrederic D Bushman
May 1, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·John J Rossi
Mar 3, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cynthia C BartholomaeManfred Schmidt
Apr 2, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Troy Brady, Frederic D Bushman
Apr 4, 2015·Viruses·Wasim AbbasGeorges Herbein
Mar 8, 2011·Expert Opinion on Biological Therapy·Grant D Trobridge
Oct 19, 2010·Life Sciences·Jui Pandhare, Chandravanu Dash
Dec 9, 2015·Annual Review of Biomedical Engineering·Melissa A KottermanDavid V Schaffer
Feb 19, 2008·Virus Research·James A Thomas, Robert J Gorelick
Sep 30, 2008·Genesis : the Journal of Genetics and Development·Shang-Hsun YangAnthony W S Chan
Sep 26, 2015·Clinical Epigenetics·Amit KumarGeorges Herbein
Jan 26, 2013·Journal of Virological Methods·Troy BradyFrederic D Bushman
Jul 31, 2012·Biochemical and Biophysical Research Communications·Tomoyuki YamaguchiHiroyuki Miyoshi
Jan 13, 2015·Current HIV/AIDS Reports·Simin D Rezaei, Paul U Cameron
May 26, 2016·FEBS Letters·Bojana Lucic, Marina Lusic
Jan 1, 2010·Viruses·Sébastien Desfarges, Angela Ciuffi
Mar 12, 2010·Expert Review of Vaccines·Mudita PinchaRenata Stripecke
Jun 23, 2006·PLoS Pathogens·Mary K LewinskiFrederic D Bushman
Dec 13, 2016·Nature Reviews. Microbiology·Marina Lusic, Robert F Siliciano
Mar 13, 2019·Nature Reviews. Drug Discovery·Marina CavazzanaEmmanuelle Six
Dec 24, 2019·Frontiers in Microbiology·Zita Kruize, Neeltje A Kootstra
Jun 25, 2020·Life Science Alliance·Donal McHughChristian Münz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.