PMID: 15385730Sep 24, 2004Paper

HIV-producing T cells in cerebrospinal fluid

Journal of Acquired Immune Deficiency Syndromes : JAIDS
Jutta K NeuenburgRobert M Grant

Abstract

In HIV-1-infected subjects, the magnitude of HIV-1 viral load in cerebrospinal fluid (CSF) correlates with the CSF white cell count. To determine whether HIV-1-producing T cells appear in CSF and whether their percentage and number correlate with viral load in CSF, we developed a flow cytometric assay that detects HIV-1-producing T cells by identifying intracellular p24 HIV-1 antigen. We found that most CSF T cells were not HIV-1 producing, even when cell-free viral load in CSF was high. Most activated T cells in CSF were also not HIV-1 producing, but the activated CD38+ CD4 T-cell fraction in CSF was independently associated with the fraction of HIV-1-producing T cells in CSF. We conclude that HIV-1-producing T cells appear in CSF and that their percentage and number correlate with cell-free viral load in CSF, even though the CSF total white cell count remains the best predictor for CSF viral load. In HIV-1 infection, CSF white cell counts seem to contain a large number of uninfected cells. White cell counts and viral load in CSF may result from systemic inflammation and immune activation.

Associated Clinical Trials

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Citations

Apr 27, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jutta K NeuenburgRichard W Price
Nov 13, 2007·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Cristiane M RochaRegina C Succi
Feb 22, 2008·Molecular Diagnosis & Therapy·Alireza MinagarPaul Shapshak

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