Oct 15, 1989

HIV promoter activity in primary antigen-specific human T lymphocytes

The Journal of Immunology : Official Journal of the American Association of Immunologists
R T Horvat, C Wood

Abstract

Human retroviruses, such as the HIV, infects human T cells, and efficient HIV replication occurs primarily in activated T cells rather than resting cells. Increased HIV production is likely caused by the activation of the retroviral promoter, and the HIV promoter may be regulated by intracellular signals induced during immune stimulation. To examine the regulation of retroviral promoter activity in normal, Ag-specific primary T lymphoblasts, a heterogeneous population of primary human T cells was transfected with either the HIV promoter or a promoter from a different retrovirus, Rous sarcoma virus (RSV) by protoplast fusion technique. Transfected T cells responded normally to Ag or mitogen stimulation, and activation of these T cells increased both the HIV and RSV promoter activity. Promoter activity was assessed by using transient expression assays after the T cells were restimulated with Ag, mitogen, or IL-2. In situ hybridization of transfected human T cells showed that 68 to 95% of activated lymphocytes expressed CAT mRNA directed by HIV or RSV. Thus, protoplast transfection of primary T cells was efficient in that the majority of cells expressed CAT message. By deletion of different regions of the HIV promoter, the enhance...Continue Reading

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Mentioned in this Paper

T-Lymphocyte
Transfection
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Protoplasm
Lymphoblast Count
Antigenic Specificity
Increased Immunologic Activity [PE]
Selfish DNA
Gene Deletion Abnormality
Promoter

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