PMID: 9436753Jan 22, 1998Paper

HIV Tat protein requirements for transactivation and repression of transcription are separable

Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association
J BrownD S Singer

Abstract

The HIV Tat protein, primarily characterized as a transcriptional activator of the viral long terminal repeat (LTR), is also a potent repressor of major histocompatibility complex (MHC) class I transcription. In the present study, we demonstrate that these two functional activities are distinct and mediated by discrete, but overlapping, structural domains of Tat. Tat repressor activity depends on C-terminal sequences, whereas transactivation depends on N-terminal sequences; both functions require core sequences. The repressor activity requires a domain encompassing the region encoded by the second exon of the Tat gene, beginning at amino acid 73, with a C-terminal limit between amino acids 80 and 83. Tat repressor function also depends on the presence of a lysine at position 41, located within the core of the protein. Tat repressor activity is independent of two N-terminal domains essential for transactivation: the acidic segment and the cysteine-rich region. Conversely, Tat transactivation is independent of the second exon-encoded region of Tat. As further support for this novel model of separable Tat functions, we show that in murine fibroblasts, Tat represses class I promoter activity, but does not transactivate the HIV LTR....Continue Reading

References

Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Jun 10, 1994·Science·D WillboldP Rösch
Mar 25, 1995·Nucleic Acids Research·J S LeeY Shi
Jun 23, 1995·The Journal of Biological Chemistry·C SchumacherH Hanafusa
Jan 1, 1994·Progress in Biophysics and Molecular Biology·A MusacchioM Saraste
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J S LeeY Shi
Jul 29, 1993·Nature·F Sauer, H Jäckle
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S C FloresJ M McCord
Jun 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·Z WangC B Morris
Jan 1, 1997·Analytical Biochemistry·T K HowcroftD S Singer

❮ Previous
Next ❯

Citations

Aug 30, 2000·European Journal of Clinical Investigation·T K Howcroft
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J D WeissmanD S Singer
Feb 15, 2001·Journal of Hematotherapy & Stem Cell Research·S Agrawal, M C Kishore
Jun 24, 2008·The Journal of Antimicrobial Chemotherapy·Inmaculada Jiménez-NácherVincent Soriano
Oct 19, 2004·Clinical Microbiology Reviews·Louis de RepentignyPaul Jolicoeur
Nov 11, 2005·Retrovirology·John Brady, Fatah Kashanchi
Nov 14, 2008·Journal of Dermatological Science·Filiberto Cedeno-LaurentJ Roberto Trujillo
Feb 27, 2004·European Journal of Clinical Investigation·M C D G HuigenH S L M Nottet
Aug 30, 2000·European Journal of Clinical Investigation·W KampH S Nottet
Oct 6, 2005·Journal of Leukocyte Biology·Alexandre IannelloAli Ahmad
Dec 12, 2007·Journal of Leukocyte Biology·Ulrich MahlknechtGeorges Herbein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.