PMID: 2496818Mar 18, 1989Paper

HIV viral sequences in seronegative people at risk detected by in situ hybridisation and polymerase chain reaction

BMJ : British Medical Journal
M PezzellaH Wigzell

Abstract

A study was conducted to assess the occurrence of latent infection with the human immunodeficiency virus (HIV) among seronegative people at high risk of infection. The presence of HIV genomes was analysed by molecular techniques in two seronegative children born to mothers infected with HIV and in three regular sexual partners of seropositive drug addicts. The adults were selected from a seronegative cohort at high risk of infection because of their sexual contacts and the children selected because of impaired growth. HIV retroviral sequences were detected in four of the five subjects directly at the cellular level by in situ hybridisation in peripheral blood mononuclear cells. HIV genomic sequences were confirmed by in vitro amplification of viral DNA with the polymerase chain reaction technique. The existence of a latent viral infection state in these seronegative subjects indicates the unreliability of standard serological analysis in people who have been in regular contact with infected patients.

Citations

Nov 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L RomanoP E Valensin
Jan 1, 1989·Current Opinion in Immunology·D Aw
Dec 13, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Lertlakana BhoopatPaul S Thorner
Oct 24, 1991·The New England Journal of Medicine·D Imagawa, R Detels
Jun 1, 1990·AIDS Research and Human Retroviruses·F CoutléeR Viscidi
Mar 1, 1991·AIDS Research and Human Retroviruses·R De SantisA Mele
Jul 1, 1992·AIDS Research and Human Retroviruses·N VoneschM Pezzella
Jan 1, 1993·Vox Sanguinis·J A Barbara, J A Garson
Dec 1, 1995·The Australian and New Zealand Journal of Surgery·D G Campbell, R D Oakeshott
Sep 1, 1991·Acta Neurologica Scandinavica·A LugaresiD Gambi
Oct 1, 1990·Genitourinary Medicine·U Desselberger, K Collingham
Jan 1, 1992·Revista Da Sociedade Brasileira De Medicina Tropical·G Brasileiro Filho, S D Pena
Dec 1, 1991·Molecular and Cellular Probes·V LombardiO Libonatti
Sep 1, 1991·Journal of Medical Virology·M PezzellaE Mannella
Feb 1, 1992·The Journal of Pathology·K A Fleming
Jan 1, 1989·Scandinavian Journal of Infectious Diseases. Supplementum·H Gaines
Jan 18, 1990·The New England Journal of Medicine·B I Eisenstein

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