PMID: 7547693Apr 1, 1995Paper

HLA-A0201 and HLA-B7 binding peptides in the EBV-encoded EBNA-1, EBNA-2 and BZLF-1 proteins detected in the MHC class I stabilization assay. Low proportion of binding motifs for several HLA class I alleles in EBNA-1

International Immunology
G StuberE Klein

Abstract

B lymphocytes immortalized with EBV in vitro, lymphoblastoid cell lines (LCL), express eight EBV-encoded proteins, EBV nuclear antigens -1 to -6 (EBNA-1 to -6), and latent membrane proteins 1 and 2 (LMP 1 and 2). After appropriate stimulations of blood lymphocytes from seropositive individuals, MHC-restricted cytotoxic T cells (CTL), which lyse LCL cells, can be generated in vitro. Such CTLs can recognize EBNA-2 to -6, and LMP 1 and 2, but not EBNA-1-derived peptides presented on the cell surfaces. We posed the question whether this exceptional feature of EBNA-1 is due to lack of MHC class I binding peptides. A computer search for 11 human leukocyte antigen (HLA) alleles showed that EBNA-1 has a lower number and lower proportion of relevant binding motifs to several alleles than EBNA-2 to -6 and LMP 1 and 2. The low motif numbers in EBNA-1 is in line with its apparent failure to generate a CTL response, and it may be the consequence of immunoselection allowing the existence of EBV genome-carrying B cells in the immunocompetent hosts. The binding capacities of synthetic peptides of EBNA-1 and -2 and of the immediate early lytic cycle protein BZLF-1 to HLA-A0201 (A2) and HLA-B7 molecules were tested in an MHC stabilization assay....Continue Reading

Citations

Sep 26, 2002·Virus Research·Victor Levitsky, Maria G Masucci
Aug 1, 1996·Current Opinion in Immunology·A B RickinsonN M Steven
Mar 1, 1997·Molecular Medicine Today·E J WiertzH L Ploegh
Apr 1, 1997·Immunology and Cell Biology·J M Davies
Jan 22, 2010·The Journal of General Virology·Neil Blake
Jul 5, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·A BeckN Corvaia
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M D CatalinaK Luzuriaga
Aug 21, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Louise C RowntreeNicole A Mifsud
Mar 1, 2020·Molecular Immunology·Emma J GrantStephanie Gras

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