PMID: 8590780Oct 1, 1995Paper

HLA-DQ screening for risk assessment of insulin dependent diabetes in northern Italy

Acta Diabetologica
V LampasonaE Bosi

Abstract

Genetic markers may be used to improve the prediction of insulin-dependent diabetes mellitus (type 1) in individuals with islet autoantibodies. In order to develop a risk assessment strategy for the Lombardy region of northern Italy based on genetic and immunological markers, we analyzed HLA DQA1 and DQB1 alleles in 60 type 1 probands and their first-degree relatives and 65 unrelated control subjects from the same area using polymerase chain reaction (PCR) and oligonucleotide probes. The major risk haplotypes were DQA1 *0501-DQB1*0201 (39.1% of diabetic vs. 8.9% of non-diabetic haplotypes) and DQA1 *0301-DQB1*0302(20% of diabetic vs 7.1% of non-diabetic haplotypes). Stratified analysis showed DQA1*0102-DQB1*0502 also to be associated with type 1 susceptibility when found together with DQA1*0501-DQB1*0201 or DQA1*0301-DQB1*0302. One type 1 patient had the type 1-protective DQA1*0102-DQB1*0602 haplotype. Overall, 88% of patients and 20% of unrelated control subjects had either DQA1*0501-DQB1*0201 or DQA1*0301-DQB1*0302 in the absence of DQA1*0102-DQB1*0602. These data suggest that typing for markers identifying these three haplotypes in the Lombardy population will achieve a sensitivity of almost 90% and exclude 80% of children f...Continue Reading

References

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Citations

Nov 22, 1997·Journal of Immunological Methods·C TibertiU Di Mario
Oct 1, 1996·Diabetes Research and Clinical Practice·J S DormanM Trucco
Feb 3, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Danny Zipris
May 17, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Claudio TibertiUmberto Di Mario
Mar 1, 2006·Journal of Autoimmunity·Sandra PiquerDaniel Laune
Apr 5, 2003·Protein Engineering·Scott E StarwaltDavid M Kranz
Dec 23, 2008·The American Journal of Gastroenterology·Andrea CassinottiGabriele Bianchi Porro

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