PMID: 7520896Apr 1, 1994Paper

HLA-DR alpha chain residues located on the outer loops are involved in nonpolymorphic and polymorphic antibody-binding epitopes

Human Immunology
X T Fu, R W Karr

Abstract

The structure-function relationships of the HLA-DR alpha chain have been analyzed by identifying DR alpha residues involved in several nonpolymorphic and polymorphic antibody epitopes. Antibody binding to transfectants expressing a WT or mutant DR alpha chain with the WT DR(beta 1*0701) chain was analyzed. Our results indicate that residues 18, 36, and 39 located on the outer loops of the DR alpha chain are critical for one or more of the epitopes recognized by the SG157, Q2/70, L243, LB3.1, D1-12, and CL413 mAbs. Similar results were obtained when the DR alpha position 18 and 39 mutants were expressed with other DR beta 1 alleles. Furthermore, residues 15 and 18 of the DR alpha chain were shown to be involved in the epitopes of two polymorphic mAbs, HU-26 and I-2, whose epitopes also include residue 4 of the corresponding DR beta chains. In addition to their involvement in antibody-binding epitopes, residues in this region on the outer surface of the DR alpha chain have also been shown to be involved in superantigen binding and presentation and T-cell recognition of foreign antigen, emphasizing the functional importance of DR alpha-chain residues located outside of the peptide-binding groove.

References

Feb 1, 1992·The Journal of Experimental Medicine·D R Karp, E O Long
Dec 1, 1992·The Journal of Experimental Medicine·P Panina-BordignonR W Karr
May 18, 1990·Cell·W G LaverS J Smith-Gill
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A HiraiwaG T Nepom
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A HermanR P Sekaly
Jul 1, 1991·The Journal of Experimental Medicine·N R NygardV L Braciale
May 11, 1990·Science·P Marrack, J Kappler
Jun 1, 1990·Science·P Marrack, J Kappler
Jul 1, 1990·The Journal of Experimental Medicine·R W KarrE Celis
Feb 1, 1989·Immunological Reviews·C A JanewayS Buxser
Aug 1, 1989·Immunology Today·B Fleischer
Feb 1, 1986·Human Immunology·P J Knudsen, J L Strominger
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P K GregersenJ Schwenzer
Nov 1, 1986·The Journal of Experimental Medicine·J Miller, R N Germain
May 1, 1981·Molecular Immunology·S CarrelR S Accolla
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S M Goyert, J Silver
Feb 1, 1983·Molecular and Cellular Biology·H Okayama, P Berg

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