HLA-E monoclonal antibodies recognize shared peptide sequences on classical HLA class Ia: relevance to human natural HLA antibodies

Molecular Immunology
Mepur H RavindranathPaul I Terasaki

Abstract

The non-classical HLA-Ib molecule, HLA-E share several peptide sequence similarities with the heavy chains of classical HLA class Ia (-B and -C) molecules. Therefore, the antibodies to HLA-E, that recognize shared sequences, may bind to HLA-Ia alleles. This hypothesis is tested by examining the affinity of HLA-E monoclonal antibodies (HLA-E-MAbs) to HLA-Ia molecules and by inhibiting the antibody binding to both HLA-E and HLA-Ia with the shared peptide sequence(s). Single recombinant HLA molecule-coated beads are used for antibody binding. The antibody binding is evaluated by measuring mean fluorescence index [MFI] with Luminex multiplex flow-cytometric technology. The peptide-inhibition experiments are carried out with synthetic shared peptides, most prevalent to HLA-E and HLA-Ia alleles. The number of HLA-Ia alleles recognized by the HLA-E-MAbs varies with the density of the antigen (quantity of antigen-coated beads) and dilution of MAb. Binding of HLA-E-MAbs to beta2 microglobulin (beta(2)m)-free HLA-Ia antigens confirms the location of the epitopes on the heavy chain (HC) of the antigens. Strikingly, the nature of alleles of HLA-Ia recognized by different HLA-E-MAbs is identical. The binding of HLA-E-MAbs to the HLA-Ia is i...Continue Reading

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D E GeraghtyH T Orr
Oct 1, 1987·The Journal of Infectious Diseases·F AmeglioR Tosi
Nov 1, 1985·Tissue Antigens·M M TongioS Mayer
Jan 1, 1973·Tissue Antigens·Z V CollinsR L Walford
Jan 1, 1994·Annual Review of Immunology·S M ShawarR R Rich
Jun 1, 1993·Scandinavian Journal of Immunology·A MarozziE Ginelli
Aug 16, 2005·Current Opinion in Immunology·Paul I Terasaki, Junchao Cai
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laurent DerréNadine Gervois
Aug 14, 2008·Transplantation·Paul I Terasaki, Junchao Cai
Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elisa Lo MonacoPatrizio Giacomini
Oct 24, 2008·Transplantation·Luis E Morales-BuenrostroJosefina Alberú

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Citations

Apr 10, 2012·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Simon F Shakar, JoAnn Lindenfeld
May 20, 2015·European Journal of Immunology·Elisa TremantePatrizio Giacomini
May 4, 2011·Vox Sanguinis·C J Brown, C V Navarrete
Sep 10, 2013·Pigment Cell & Melanoma Research·Elisa TremantePatrizio Giacomini
Oct 10, 2013·International Journal of Cancer. Journal International Du Cancer·Toshiyuki SasakiVadim Jucaud
Mar 26, 2015·Journal of the American Society of Nephrology : JASN·Ana Konvalinka, Kathryn Tinckam
Apr 23, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Mepur H RavindranathShahab Asgharzadeh
Jul 9, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mepur H RavindranathPaul I Terasaki
May 16, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Linda BorstThorbald van Hall

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