HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration

Journal of Clinical Medicine
Sunao SugitaMasayo Takahashi

Abstract

Immune attacks are key issues for cell transplantation. To assess the safety and the immune reactions after iPS cells-derived retinal pigment epithelium (iPS-RPE) transplantation, we transplanted HLA homozygote iPS-RPE cells established at an iPS bank in HLA-matched patients with exudative age-related macular degeneration. In addition, local steroids without immunosuppressive medications were administered. We monitored immune rejections by routine ocular examinations as well as by lymphocytes-graft cells immune reaction (LGIR) tests using graft RPE and the patient's blood cells. In all five of the cases that underwent iPS-RPE transplantation, the presence of graft cells was indicated by clumps or an area of increased pigmentation at 6 months, which became stable with no further abnormal growth in the graft during the 1-year observation period. Adverse events observed included corneal erosion, epiretinal membrane, retinal edema due to epiretinal membrane, elevated intraocular pressure, endophthalmitis, and mild immune rejection in the eye. In the one case exhibiting positive LGIR tests along with a slight fluid recurrence, we administrated local steroid therapy that subsequently resolved the suspected immune attacks. Although th...Continue Reading

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Citations

Sep 19, 2020·Development·Hideyuki Okano, Doug Sipp
Jan 23, 2021·Progress in Retinal and Eye Research·Sunao SugitaMasayo Takahashi
Oct 28, 2020·International Journal of Molecular Sciences·Ayaka EdoMasayo Takahashi
Jan 23, 2021·Genes·Marta García-LópezM Esther Gallardo
Apr 4, 2021·International Journal of Molecular Sciences·Masaaki IshidaMasayo Takahashi
Apr 27, 2021·Frontiers in Immunology·Charlotte FlahouNaoshi Sugimoto
May 1, 2021·Journal of Clinical Medicine·Tadao MaedaMasayo Takahashi
May 19, 2021·Orphanet Journal of Rare Diseases·Ana B Garcia-DelgadoBerta de la Cerda
May 26, 2021·Expert Opinion on Emerging Drugs·Masumi G AsahiRon P Gallemore
Jan 10, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Eléanor LuceAnne Dubart-Kupperschmitt
Aug 19, 2021·The FEBS Journal·Hiraku Tsujimoto, Kenji Osafune
Oct 3, 2020·Cell Stem Cell·Shinya Yamanaka
Oct 15, 2021·Médecine sciences : M/S·Eléanor LuceJean-Charles Duclos-Vallée

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Methods Mentioned

BETA
xenografts
genotyping
PCR
ELISA
confocal microscopy
RSA
flow cytometry

Software Mentioned

stats
em
R
haplo

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