PMID: 20103991Jan 28, 2010Paper

Hlcyst-1 and Hlcyst-2 are potential inhibitors of HlCPL-A in the midgut of the ixodid tick Haemaphysalis longicornis

The Journal of Veterinary Medical Science
Kayoko YamajiKozo Fujisaki

Abstract

Although the actions of cysteine proteases are controlled in part by endogenous tight-binding cysteine protease inhibitors from the cystatin superfamily, regulatory mechanisms used by ticks to control protease activities are unknown. We report here the interaction of 2 endogenous midgut cysteine protease inhibitors, Hlcyst-1 and Hlcyst-2, with an endogenous midgut cysteine protease, HlCPL-A in Haemaphysalis longicornis. In vitro inhibition assays demonstrated that the hydrolytic activity of HlCPL-A was inhibited by Hlcyst-1 and Hlcyst-2 in dose dependent manner. Immunofluorescent studies revealed that Hlcyst-1 and Hlcyst-2 are co-localized with HlCPL-A in the epithelial cells of the midgut. The hemoglobin degradation activity of HlCPL-A was dose-dependently inhibited by Hlcyst-1 and Hlcyst-2. These results strongly indicate that, Hlcyst-1 and Hlcyst-2 are possible inhibitor of HlCPL-A and play a key role in regulatory mechanisms of hemoglobin degradation process in ticks.

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Citations

Dec 16, 2010·Parasites & Vectors·Zdeněk FrantaPetr Kopáček
Oct 12, 2012·Experimental & Applied Acarology·Adriana Mércia Guaratini IbelliAlbert Mulenga
Sep 17, 2013·Ticks and Tick-borne Diseases·Luís F PariziItabajara da Silva Vaz
Dec 26, 2012·Ticks and Tick-borne Diseases·Saiki ImamuraKazuhiko Ohashi
Aug 9, 2011·International Journal for Parasitology·Zdenek FrantaPetr Kopacek
Jul 21, 2015·Experimental & Applied Acarology·Yujian WangJinlin Zhou
Feb 19, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Kayoko YamajiNaotoshi Tsuji
Jun 1, 2012·Ticks and Tick-borne Diseases·Alexandra SchwarzMichalis Kotsyfakis
Apr 19, 2015·Parasites & Vectors·Luís Fernando PariziItabajara da Silva Vaz
Nov 8, 2017·Medical and Veterinary Entomology·L F PariziI DA Silva Vaz
Dec 14, 2017·Scientific Reports·Jana IlgováMartin Kašný
Jun 15, 2017·Frontiers in Cellular and Infection Microbiology·Jindřich ChmelařMichail Kotsyfakis
Feb 6, 2020·Ticks and Tick-borne Diseases·Stephen LuAparecida S Tanaka

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