HMGB1 Knockout Decreases Kaposi's Sarcoma-Associated Herpesvirus Virion Production in iSLK BAC16 Cells by Attenuating Viral Gene Expression.

Journal of Virology
Su-Kyung KangMyung-Shin Lee

Abstract

Multiple host proteins affect the gene expression of Kaposi's sarcoma-associated herpesvirus (KSHV) during latent and lytic replication. High-mobility group box 1 (HMGB1) serves as a highly conserved chromosomal protein inside the cell and a prototypical damage-associated molecular pattern molecule outside the cell. HMGB1 has been shown to play a pathogenic role in viral infectious diseases and to regulate the lytic replication of KSHV. However, its functional effects on the KSHV life cycle in KSHV-infected cells have not been fully elucidated. Here, we explored the role of intracellular and extracellular HMGB1 in KSHV virion production by employing CRISPR/Cas9-mediated HMGB1 knockout in the KSHV-producing iSLK BAC16 cell line. Intracellular HMGB1 formed complexes with various proteins, and the abundance of HMGB1-interacting proteins changed during latent and lytic replication. Moreover, extracellular HMGB1 was found to enhance lytic replication by phosphorylating JNK. Of note, the expression of viral genes was attenuated during lytic replication in HMGB1 knockout iSLK BAC16 cells, with significantly decreased production of infectious virions compared to that of wild-type cells. Collectively, our results demonstrate that HMGB1 ...Continue Reading

References

May 29, 2000·Molecular and Cellular Biology·K B EllwoodM Carey
May 25, 2002·Molecular and Cellular Biology·Katherine MitsourasMichael Carey
May 28, 2004·Journal of Virology·Rafael E LunaKonstantin G Kousoulas
Apr 11, 2006·The Journal of General Virology·Patrick W FordShaw M Akula
Mar 3, 2010·Annual Review of Immunology·Gary P SimsAnthony J Coyle
Jan 12, 2011·Annual Review of Immunology·Ulf Andersson, Kevin J Tracey
Feb 2, 2011·PloS One·Chloé BordeJoël Gozlan
Feb 2, 2012·Nature Reviews. Rheumatology·Helena Erlandsson HarrisDavid S Pisetsky
Jun 15, 2012·Journal of Virology·Dorothée MoisyJean-Luc Jestin
Nov 20, 2013·Acta Biochimica Et Biophysica Sinica·Cailian ZhangJinran Zhang
Jan 17, 2015·Viruses·Pravinkumar PurushothamanSubhash C Verma
Dec 31, 2015·Proceedings of the National Academy of Sciences of the United States of America·Anna BangertZiya Kaya
Sep 24, 2016·Current Opinion in HIV and AIDS·Priscila H GoncalvesRobert Yarchoan
May 6, 2017·Frontiers in Microbiology·Kawalpreet K Aneja, Yan Yuan
Jul 15, 2020·Journal of Hematology & Oncology·Shunling YuanJi Zhang

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE172275
GSE157275

Methods Mentioned

BETA
PCR
reverse transcription-PCR
Illumina Sequencing
transfection
RNA-seq
Flow cytometry
fluorescence microscopy
immunoprecipitation
histone acetylation
electrophoresis

Software Mentioned

Image Lab
exactTest
StringTie
Metascape
bowtie
edgeR
Morpheus
InCyte

Related Concepts

Related Feeds

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
I Maruyama
Nihon rinsho. Japanese journal of clinical medicine
I Maruyama, S Yamada
Journal of Cellular Physiology
Jiaming XueHaining Yang
Journal of Leukocyte Biology
Vilma UrbonaviciuteReinhard E Voll
Biochimica Et Biophysica Acta
Ulf Andersson, Helena Harris
© 2021 Meta ULC. All rights reserved