HMGB1 protein inhibits DNA replication in vitro: a role of the acetylation and the acidic tail

The International Journal of Biochemistry & Cell Biology
Dessislava TopalovaE A Pasheva

Abstract

The high mobility group box (HMGB) 1 protein is a very abundant and conserved protein that is implicated in many key cellular events but its functions within the nucleus remain elusive. The role of this protein in replication of closed circular DNA containing a eukaryotic origin of replication has been studied in vitro by using native and recombinant HMGB1 as well as various modified HMGB1 preparations such as truncated protein, lacking its C-terminal tail, in vivo acetylated protein, and recombinant HMGB1 phosphorylated in vitro by protein kinase C (PKC). Native HMGB1 extracted from tumour cells inhibits replication and this effect is reduced upon acetylation and completely abolished upon removal of the acidic C-terminal tail. Recombinant HMGB1, however, fails to inhibit replication but it acquires such a property following in vitro phosphorylation by PKC.

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Citations

Oct 26, 2010·Antioxidants & Redox Signaling·Daolin TangMichael T Lotze
Jan 25, 2011·Journal of Biochemistry·Iva UgrinovaEvdokia A Pasheva
Mar 19, 2009·Journal of Translational Medicine·Louis J SparveroMichael T Lotze
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Mar 14, 2013·Protein & Cell·Ben LuKevin J Tracey
Sep 12, 2018·Hepatology Communications·Harriet GaskellNatalia Nieto

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