HMGB1 Translocation in Neurons after Ischemic Insult: Subcellular Localization in Mitochondria and Peroxisomes.

Cells
Dengli WangMasahiro Nishibori

Abstract

High mobility group box-1 (HMGB1), a nonhistone chromatin DNA-binding protein, is released from neurons into the extracellular space under ischemic, hemorrhagic, and traumatic insults. However, the details of the time-dependent translocation of HMGB1 and the subcellular localization of HMGB1 through the release process in neurons remain unclear. In the present study, we examined the subcellular localization of HMGB1 during translocation of HMGB1 in the cytosolic compartment using a middle cerebral artery occlusion and reperfusion model in rats. Double immunofluorescence microscopy revealed that HMGB1 immunoreactivities were colocalized with MTCO1(mitochondrially encoded cytochrome c oxidase I), a marker of mitochondria, and catalase, a marker of peroxisomes, but not with Rab5/Rab7 (RAS-related GTP-binding protein), LC3A/B (microtubule-associated protein 1 light chain 3), KDEL (KDEL amino acid sequence), and LAMP1 (Lysosomal Associated Membrane Protein 1), which are endosome, phagosome, endoplasmic reticulum, and lysosome markers, respectively. Immunoelectron microscopy confirmed that immune-gold particles for HMGB1 were present inside the mitochondria and peroxisomes. Moreover, HMGB1 was found to be colocalized with Drp1 (Dynam...Continue Reading

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Citations

Jun 27, 2020·Frontiers in Immunology·Man Sup KwakJeon-Soo Shin
Feb 10, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kazuki HatayamaBarbara S Stonestreet
Dec 17, 2020·Cells·Masahiro NishiboriHidenori Wake
Mar 7, 2021·International Journal of Molecular Sciences·Ji-Eun KimTae-Cheon Kang

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Methods Mentioned

BETA
acetylation

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