HMP-1/α-catenin promotes junctional mechanical integrity during morphogenesis

PloS One
Thanh T K Vuong-BrenderMichel Labouesse

Abstract

Adherens junctions (AJs) are key structures regulating tissue integrity and maintaining adhesion between cells. During morphogenesis, junctional proteins cooperate closely with the actomyosin network to drive cell movement and shape changes. How the junctions integrate the mechanical forces in space and in time during an in vivo morphogenetic event is still largely unknown, due to a lack of quantitative data. To address this issue, we inserted a functional Fluorescence Resonance Energy Transfer (FRET)-based force biosensor within HMP-1/α-catenin of Caenorhabditis elegans. We find that the tension exerted on HMP-1 has a cell-specific distribution, is actomyosin-dependent, but is regulated differently from the tension on the actin cortex during embryonic elongation. By using time-lapse analysis of mutants and tissue-specific rescue experiments, we confirm the role of VAB-9/Claudin as an actin bundle anchor. Nevertheless, the tension exerted on HMP-1 did not increase in the absence of VAB-9/Claudin, suggesting that HMP-1 activity is not upregulated to compensate for loss of VAB-9. Our data indicate that HMP-1 does not modulate HMR-1/E-cadherin turnover, is required to recruit junctional actin but not stress fiber-like actin bundle...Continue Reading

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Citations

Mar 19, 2020·Journal of Developmental Biology·Cátia A Carvalho, Limor Broday
Nov 16, 2018·Biophysics Reviews·Justin Parreno, Velia M Fowler
Jun 7, 2021·Current Opinion in Cell Biology·Kendelle J MurphyPaul Timpson
May 12, 2021·G3 : Genes - Genomes - Genetics·Elizabeth D DrewnikPaul E Mains

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Methods Mentioned

BETA
force measurements
biosensor
Fluorescence
FRET
transgenic
fluorescence microscopy
tension
PCR

Software Mentioned

PixFRET
ImageJ
Huygens Essential
Mathlab
Matlab
Scientific Volume Imaging
Metamorph

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