HNF1 regulates critical processes in the human epididymis epithelium

Molecular and Cellular Endocrinology
James A BrowneAnn Harris

Abstract

The luminal environment of the epididymis participates in sperm maturation and impacts male fertility. It is dependent on the coordinated expression of many genes encoding proteins with a role in epithelial transport. We identified cis-regulatory elements for critical genes in epididymis function, by mapping open chromatin genome-wide in human epididymis epithelial (HEE) cells. Bioinformatic predictions of transcription factors binding to the regulatory elements suggested an important role for hepatocyte nuclear factor 1 (HNF1) in the transcriptional program of these cells. Chromatin immunoprecipitation and deep sequencing (ChIP-seq) revealed HNF1 target genes in HEE cells. In parallel, the contribution of HNF1 to the transcriptome of HEE cells was determined by RNA-seq, following siRNA-mediated depletion of both HNF1α and HNF1β transcription factors. Repression of these factors caused differential expression of 1892 transcripts (902 were downregulated and 990 upregulated) in comparison to non-targeting siRNAs. Differentially expressed genes with HNF1 ChIP-seq peaks within 20 kb were subject to gene ontology process enrichment analysis. Among the most significant processes associated with down-regulated genes were epithelial tr...Continue Reading

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Citations

May 3, 2019·Andrology·J A BrowneA Harris
May 21, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alekh ParanjapyeAnn Harris
Jul 22, 2020·Journal of Cellular and Molecular Medicine·Jenny L KerschnerAnn Harris
Jul 5, 2021·The Journal of Biological Chemistry·Alekh ParanjapyeAnn Harris
Oct 5, 2021·The Biochemical Journal·Monali NandyMazumdarAnn Harris

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