Hoechst 33342 staining coupled with conventional histological technique.

Stain Technology
W Sawicki, S Moskalewski

Abstract

Hoechst 33342 was injected either intravenously or intraperitoneally into mice which were killed 1 or 24 hr or 7, 14 or 28 days later. Various organs were fixed and paraffin embedded. Visual inspection showed that independently of the route of dye administration or survival time, distinct fluorescence of nuclei was observed in organs other than cerebral cortex. Even formic acid decalcification of bone failed to abolish the fluorescence of osteocytes. In vivo staining with Hoechst 33342 is proposed as an alternative for staining after sectioning. Cells from spleens of Hoechst 33342-injected mice or stained in vitro were injected intramuscularly into mice. Hoechst 33342-stained splenocytes could be found in deparaffinized sections at the site of injection 24 hr later.

References

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Citations

Feb 1, 1991·Journal of Neuroscience Methods·U Sprick
May 6, 2004·Archives of Histology and Cytology·Keisuke KawasakiHajime Inoue
Jul 6, 2014·BioMed Research International·V Ganga PrasadRekha R Shenoy
Jul 1, 1990·The Anatomical Record·W Sawicki, E T Mystkowska
May 31, 2011·Morphologie : Bulletin De L'Association Des Anatomistes·P RusseD Chappard
Feb 23, 2017·Annals of Biomedical Engineering·Yulia Merkher, Daphne Weihs
Nov 7, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Xiaomin SunQiqing Zhang

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