Hog1 targets Whi5 and Msa1 transcription factors to downregulate cyclin expression upon stress

Molecular and Cellular Biology
Alberto González-NovoFrancesc Posas

Abstract

Yeast cells have developed complex mechanisms to cope with extracellular insults. An increase in external osmolarity leads to activation of the stress-activated protein kinase Hog1, which is the main regulator of adaptive responses, such as gene expression and cell cycle progression, that are essential for cellular survival. Upon osmostress, the G1-to-S transition is regulated by Hog1 through stabilization of the cyclin-dependent kinase inhibitor Sic1 and the downregulation of G1 cyclin expression by an unclear mechanism. Here, we show that Hog1 interacts with and phosphorylates components of the core cell cycle transcriptional machinery such as Whi5 and the coregulator Msa1. Phosphorylation of these two transcriptional regulators by Hog1 is essential for inhibition of G1 cyclin expression, for control of cell morphogenesis, and for maximal cell survival upon stress. The control of both Whi5 and Msa1 by Hog1 also revealed the necessity for proper coordination of budding and DNA replication. Thus, Hog1 regulates G1 cyclin transcription upon osmostress to ensure coherent passage through Start.

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Citations

Aug 22, 2016·Current Genetics·Shawna Miles, Linda Breeden
Oct 14, 2016·Frontiers in Microbiology·Ramon GonzalezEva Valero
Nov 21, 2017·PLoS Genetics·Andrea SilvaEulàlia de Nadal
May 20, 2018·Applied and Environmental Microbiology·Hui LiuLiming Liu
Apr 21, 2016·Nature Communications·Pasquale PalumboLilia Alberghina
Apr 9, 2020·Proceedings of the National Academy of Sciences of the United States of America·Silvia TognettiFrancesc Posas
Aug 26, 2017·Nature Communications·Mercè Gomar-AlbaJ Carlos Igual
Apr 17, 2019·Life Science Alliance·David F MorenoMartí Aldea
Jun 19, 2019·Cell Communication and Signaling : CCS·Marion JanschitzWolfgang Reiter
Sep 26, 2020·Scientific Reports·Diego VelázquezJoaquín Ariño
May 31, 2018·NPJ Systems Biology and Applications·Aouefa AmoussouviEdda Klipp
Jan 23, 2021·The Journal of Cell Biology·Galal YahyaMartí Aldea
Dec 12, 2021·Critical Reviews in Microbiology·Hajar YaakoubNicolas Papon

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