PMID: 9421521Feb 28, 1998Paper

Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2

Nucleic Acids Research
M OramI R Tsaneva

Abstract

A novel Holliday junction resolving activity has been identified in fractionated cell extracts of the fission yeast Schizosaccharomyces pombe . The enzyme catalyses endonucleolytic cleavage of Holliday junction-containing chi DNA and synthetic four-way DNA junctions. The activity cuts with high specificity a synthetic four-way junction containing a 12 bp core of homologous sequences but has no activity on another four-way junction (with a fixed crossover point), a three-way junction, linear duplex DNA or duplex DNA containing six mismatched nucleotides in the centre. The major cleavage sites map as single nicks in the vicinity of the crossover point, 3' of a thymidine residue. These data indicate that the activity has a strong DNA structure selectivity as well as a limited sequence preference; features similar to the Holliday junction resolving enzymes RuvC of Escherichia coli and the mitochondrial CCE1 (cruciform-cuttingenzyme 1) of Saccharomyces cerevisiae. A putative homologue of CCE1 in S.pombe (YDC2_SCHPO) has been identified through a search of the sequence database. The open reading frame of this gene has been cloned and the encoded protein, YDC2, expressed in E.coli . The purified recombinant YDC2 exhibits Holliday junc...Continue Reading

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Citations

Mar 11, 2000·Molecular Biology of the Cell·E B AlbrechtT E Patterson
Mar 29, 2008·PLoS Genetics·Zeynep BaharogluBénédicte Michel
Apr 5, 2001·Journal of Molecular Biology·A C DéclaisD M Lilley
Jun 26, 2015·FEMS Microbiology Reviews·Adrianna SkonecznaMarek Skoneczny
Dec 13, 2006·Journal of Molecular Biology·Teena PuriIrina R Tsaneva
Oct 17, 1998·Molecular Cell·S M RoeL H Pearl
Nov 24, 2004·The Journal of Biological Chemistry·Cyril V PrivezentzevIrina R Tsaneva
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Stephen C West
May 16, 2009·Biochemical Society Transactions·Stephen C West
Dec 18, 1998·The Journal of Biological Chemistry·M C Whitby, J Dixon

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