Holliday junction resolvases mediate chloroplast nucleoid segregation

Science
Yusuke KobayashiYoshiki Nishimura

Abstract

Holliday junctions, four-stranded DNA structures formed during homologous recombination, are disentangled by resolvases that have been found in prokaryotes and eukaryotes but not in plant organelles. Here, we identify monokaryotic chloroplast 1 (MOC1) as a Holliday junction resolvase in chloroplasts by analyzing a green alga Chlamydomonas reinhardtii mutant defective in chloroplast nucleoid (DNA-protein complex) segregation. MOC1 is structurally similar to a bacterial Holliday junction resolvase, resistance to ultraviolet (Ruv) C, and genetically conserved among green plants. Reduced or no expression of MOC1 in Arabidopsis thaliana leads to growth defects and aberrant chloroplast nucleoid segregation. In vitro biochemical analysis and high-speed atomic force microscopic analysis revealed that A. thaliana MOC 1 (AtMOC1) binds and cleaves the core of Holliday junctions symmetrically. MOC1 may mediate chloroplast nucleoid segregation in green plants by resolving Holliday junctions.

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Citations

Oct 16, 2019·Nature Chemical Biology·Huajian LinZhonghui Lin
Jun 4, 2018·Plant & Cell Physiology·Wataru Sakamoto, Tsuneaki Takami
Sep 25, 2019·Plants·Stewart A MorleyBrent L Nielsen
Jan 18, 2020·International Journal of Molecular Sciences·Nicolas ChevignyJosé Manuel Gualberto
Oct 3, 2018·Communications Biology·Yoshitaka KamimuraYoshiki Nishimura
May 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Mari TakusagawaYoshiki Nishimura
May 31, 2019·Molecular Phylogenetics and Evolution·Deise J P GonçalvesRobert K Jansen

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