Hollow fiber-based liquid phase microextraction with factorial design optimization and gas chromatography-tandem mass spectrometry for determination of cannabinoids in human hair

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Elissandro Soares EmídioHaroldo Silveira Dórea

Abstract

A new method, based on hollow fiber liquid-phase microextraction (HF-LPME) and gas chromatography-tandem mass spectrometry (GC-MSMS), was developed for determination of Delta(9)-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in samples of human hair. Since hair is a solid matrix, the samples were subjected to alkaline digestion using NaOH. The aqueous solutions obtained were extracted using a 6cm polypropylene fiber (600microm i.d., 200microm wall thickness, 0.2microm pore size) for each extraction. A 2(5-1) fractional factorial design for screening, and a central composite design for optimization of significant variables, was applied during development of the extraction method. The variables evaluated were the type of extraction solvent, pH, stirring speed, extraction time, and acceptor phase volume. The optimized conditions for the proposed extraction procedure were 10mg of hair sample; 20microL of butyl acetate; aqueous (pH 14) donor phase containing 6.8% NaCl; 600rpm stirring speed; 20min extraction time. A linear response was obtained in the ranges 1-500pgmg(-1) (CBD and CBN) and 20-500pgmg(-1) (THC), with regression coefficients >0.99. Precision, determined as the relative standard deviation, was 3.3-8...Continue Reading

References

Nov 17, 1995·Journal of Chromatography. B, Biomedical Applications·V CirimeleP Mangin
Jan 1, 1997·International Journal of Legal Medicine·C JuradoM Repetto
Feb 18, 1999·Journal of Analytical Toxicology·S Strano-Rossi, M Chiarotti
Feb 26, 2000·Forensic Science International : Synergy·F Sporkert, F Pragst
Sep 5, 2002·Forensic Science International : Synergy·Maria João BaptistaDuarte Nuno Vieira
May 14, 2003·Forensic Science International : Synergy·Frank MusshoffBurkhard Madea
Sep 9, 2008·Phytochemistry·Mohamed M RadwanSamir A Ross

❮ Previous
Next ❯

Citations

Sep 9, 2011·Bioanalysis·Victoria SamanidouKonstantinos Rentifis
Aug 11, 2012·Bioanalysis·Mário BarrosoEugenia Gallardo
Jan 12, 2013·Journal of Pharmaceutical and Biomedical Analysis·Laura MercoliniMaria Augusta Raggi
Jan 22, 2015·Analytica Chimica Acta·Susanna VogliardiDonata Favretto
Jun 28, 2016·Journal of Analytical Toxicology·Sonja HeinlFreidoon Erdmann
Jan 22, 2019·Bioanalysis·Gabriela de Oliveira SilveiraMauricio Yonamine
May 28, 2019·Analytical and Bioanalytical Chemistry·Pedro Rafael da SilvaFernando José Malagueño de Santana
Feb 13, 2020·Mass Spectrometry Reviews·Scott A BordenPierangela Palma
May 25, 2011·Journal of Separation Science·Morteza MoradiTahmineh Baheri
Mar 18, 2021·The Analyst·Georgina SauzierSimon W Lewis
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samir M AhmadJosé M F Nogueira
Mar 10, 2016·Journal of the American Society for Mass Spectrometry·Elizabeth S HechtDavid C Muddiman
May 3, 2011·Analytical Chemistry·T A BrettellJ R Almirall
Nov 9, 2019·Chemical Research in Toxicology·Carla FerreiraAlexandre Quintas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved