Hollow Nanoneedle-Electroporation System To Extract Intracellular Protein Repetitively and Nondestructively

ACS Sensors
Gen HeXi Xie

Abstract

Techniques used to understand the dynamic expression of intracellular proteins are critical in both fundamental biological research and biomedical engineering. Various methods for analyzing proteins have been developed, but these methods require the extraction of intracellular proteins from the cells resulting in cell lysis and subsequent protein purifications from the lysate, which limits the potential of repetitive extraction from the same set of viable cells to track dynamic intracellular protein expression. Therefore, it is crucial to develop novel methods that enable nondestructive and repeated extraction of intracellular proteins. This work reports a hollow nanoneedle-electroporation system for the repeated extraction of intracellular proteins from living cells. Hollow nanoneedles with ∼450 nm diameter were fabricated by a material deposition and etching process, followed by integration with a microfluidic device. Long-lasting electrical pulses were coupled with the nanoneedles to permeate the cell membrane, allowing intracellular contents to diffuse into the microfluidic channels located below the cells via hollow nanoneedles. Using lactate dehydrogenase B (LDHB) as the model intracellular protein, the nanoneedle-electro...Continue Reading

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Citations

Jan 17, 2020·Advanced Materials·Stuart G HigginsMolly M Stevens
Aug 4, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiangling LiFanmao Liu
Feb 4, 2021·Microsystems & Nanoengineering·An'an ShengHuanyu Cheng
Feb 19, 2021·Journal of Nanobiotechnology·Roey ElnathanNicolas H Voelcker
Aug 25, 2021·Nature Protocols·Ciro ChiappiniRoey Elnathan
Nov 8, 2019·ACS Applied Materials & Interfaces·Rui WenXi Xie
Sep 2, 2020·Journal of the American Chemical Society·Stella AslanoglouRoey Elnathan
Jan 21, 2022·Chemical Reviews·Kevin S ZhangSindy K Y Tang

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