Homeobox genes are differentially expressed in macrovascular human umbilical vein endothelial cells and microvascular placental endothelial cells

Placenta
P MurthiB Kalionis

Abstract

Angiogenesis is fundamental to normal placental development. Aberrant angiogenesis within the placental terminal villi is a characteristic of significant placental pathologies and includes structural and vascular abnormalities as well as altered endothelial cell function, which substantially impacts on maternal-fetal exchange. Homeobox gene transcription factors regulate vascular development in embryonic and adult tissues, but their role in the placental microvasculature is not well known. In this study, we isolated and enriched human placental microvascular endothelial cells (PLEC) by a perfusion-based method and compared homeobox gene expression between PLEC and macrovascular human umbilical vein endothelial cells (HUVEC). Reverse transcriptase PCR detected mRNA expression of homeobox genes DLX3, DLX4, MSX2, GAX and HLX1 in both PLEC and HUVEC. DLX4 and HLX1 have not been previously detected in PLEC and with the exception of GAX, none of these homeobox genes have been previously identified in HUVEC. There was lower expression of HLX1 mRNA in HUVEC compared with PLEC. Using real-time PCR analysis PLEC HLX1 mRNA expression relative to housekeeping gene GAPDH was 0.9+/-0.06 fold of the calibrator (n=6) versus 0.2+/-0.06 (n=6) fo...Continue Reading

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Citations

Nov 25, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Amy ChuiPadma Murthi
Nov 22, 2011·Current Diabetes Reports·M GausterU Hiden
Apr 12, 2008·Reproduction, Fertility, and Development·Gayathri RajaramanBill Kalionis
Aug 22, 2012·Fetal Diagnosis and Therapy·Padma MurthiFabricio Da Silva Costa
Oct 21, 2010·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Louiza BelkacemiMichael G Ross
Dec 17, 2009·The American Journal of Pathology·Gayathri RajaramanBill Kalionis
Jun 24, 2014·BioMed Research International·Nadia AlfaidySophie Brouillet
Aug 20, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Tilini GunatillakePadma Murthi
Dec 8, 2017·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Nicola YuenPadma Murthi
Apr 8, 2017·International Journal of Endocrinology·Ana Silvia CorlanMarius Raica
Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Antonietta Rosa SiliniOrnella Parolini

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