Homeogene emx1 is required for nephron distal segment development in zebrafish

Scientific Reports
Elvin E MoralesRebecca A Wingert

Abstract

Vertebrate kidneys contain nephron functional units where specialized epithelial cell types are organized into segments with discrete physiological roles. Many gaps remain in our understanding of how segment regions develop. Here, we report that the transcription factor empty spiracles homeobox gene 1 (emx1) is a novel nephron segment regulator during embryonic kidney development in zebrafish. emx1 loss of function altered the domains of distal segments without changes in cell turnover or traits like size and morphology, indicating that emx1 directs distal segment fates during nephrogenesis. In exploring how emx1 influences nephron patterning, we found that retinoic acid (RA), a morphogen that induces proximal and represses distal segments, negatively regulates emx1 expression. Next, through a series of genetic studies, we found that emx1 acts downstream of a cascade involving mecom and tbx2b, which encode essential distal segment transcription factors. Finally, we determined that emx1 regulates the expression domains of irx3b and irx1a to control distal segmentation, and sim1a to control corpuscle of Stannius formation. Taken together, our work reveals for the first time that emx1 is a key component of the pronephros segmentat...Continue Reading

References

Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Aug 25, 1996·Developmental Biology·M QiuJ L Rubenstein
Feb 7, 1998·Anatomy and Embryology·M PellegriniA Forabosco
Jul 25, 2000·Trends in Neurosciences·C Cecchi, E Boncinelli
Oct 31, 2001·Development Genes and Evolution·C W ChengS H Cheng
Nov 16, 2001·Cerebral Cortex·C H ChanJ G Parnavelas
Feb 19, 2003·Development Genes and Evolution·Esther CampMichael Lardelli
Mar 6, 2003·Gene Expression Patterns : GEP·Atsuo Kawahara, Igor B Dawid
May 3, 2003·The Journal of Clinical Endocrinology and Metabolism·Nathalie MassinPhilippe Touraine
Apr 13, 2005·Current Biology : CB·Michael R ElizondoDavid M Parichy
Jul 7, 2006·Annual Review of Cell and Developmental Biology·Gregory R Dressler
Sep 19, 2007·Genes & Development·Luca ReggianiAndré W Brändli
Sep 20, 2007·Nature Reviews. Genetics·Andreas Schedl
Feb 5, 2008·Experimental Hematology·Jenna L GallowayLeonard I Zon
Feb 22, 2008·Journal of the American Society of Nephrology : JASN·Andrew P McMahonUNKNOWN GUDMAP project
Mar 7, 2008·Kidney International·R A Wingert, A J Davidson
Aug 4, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Gudrun ViktorinMonte Westerfield
Apr 7, 2011·Developmental Biology·Claudia LengerkeAlan J Davidson
Apr 27, 2011·Current Opinion in Nephrology and Hypertension·Lwaki EbarasiKarl Tryggvason
Jul 16, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rebecca A Wingert, Alan J Davidson
Jan 23, 2013·Nature Reviews. Nephrology·Paola RomagnaniGiuseppe Remuzzi
Mar 28, 2013·The Journal of Clinical Endocrinology and Metabolism·Flavia Amanda Costa-BarbosaWilliam F Crowley
Sep 10, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Gary F Gerlach, Rebecca A Wingert
Nov 26, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Michael A Pickart, Eric W Klee
Jun 13, 2014·Genesis : the Journal of Genetics and Development·Paul T Kroeger, Rebecca A Wingert
Aug 1, 2014·Journal of Visualized Experiments : JoVE·Christina N ChengRebecca A Wingert
Aug 26, 2014·Journal of Visualized Experiments : JoVE·Kristen K McCampbellRebecca A Wingert
Nov 20, 2014·Journal of Visualized Experiments : JoVE·Shahram Jevin PoureetezadiRebecca A Wingert
Dec 3, 2014·Gene Expression Patterns : GEP·Robert McKeeRebecca A Wingert
Jun 20, 2015·Stem Cells International·Kristen K McCampbellRebecca A Wingert
Sep 18, 2015·Cells·Audrey Desgrange, Silvia Cereghini
Nov 5, 2015·Development·Jonathan W C LimLinda J Richards
May 12, 2016·Kidney International·Shahram Jevin Poureetezadi, Rebecca A Wingert
Jul 9, 2016·Genesis : the Journal of Genetics and Development·Amanda N MarraRebecca A Wingert

❮ Previous
Next ❯

Citations

Nov 27, 2018·ELife·Joseph M ChambersRebecca A Wingert
Jun 5, 2019·Development·Brooke E ChambersRebecca A Wingert
Oct 24, 2020·Tissue Barriers·Joseph M Chambers, Rebecca A Wingert
Nov 26, 2020·Scientific Reports·Frédéric JehlSandrine Lagarrigue
Oct 9, 2020·Development·Brooke E ChambersRebecca A Wingert
Mar 27, 2021·Journal of the American Society of Nephrology : JASN·Lihe ChenMark A Knepper

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
chemical treatments

Software Mentioned

Nikon Elements
FIJI
WISH

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.