Homocitrate synthase expression and lysine content in fruiting body of different developmental stages in Flammulina velutipes

Current Microbiology
Fang LiuBao-Gui Xie

Abstract

Homocitrate synthase (EC 2.3.3.14) regulates the first step of fungal lysine biosynthesis. The gene encoding homocitrate synthase was identified in whole genomic sequencing of Flammulina velutipes and contains seven introns. The homocitrate synthase gene of F. velutipes strain W23 (Fvhcs) is 1780 bp in length and encodes a 464 amino acid protein with a predicted molecular weight 50.7 kDa. Phylogenetic analysis of Fvhcs and other homocitrate synthase proteins from diverse fungi produced a topology congruent with the current best estimate of organismal phylogeny. Analysis of protein domains by InterProScan and a motif search found that Fvhcs gene encodes homocitrate synthase protein conserved across Agaricomycotina. In addition, we sequenced the transcriptome of different developmental stages and structures of the fruiting body to analyze the expression levels of the Fvhcs gene. The data showed a correlation between Fvhcs gene expression and lysine values in different developmental stages and structures of F. velutipes.

References

Jan 1, 1978·Annual Review of Biochemistry·H E Umbarger
Jan 1, 1985·Critical Reviews in Microbiology·J K Bhattacharjee
Dec 1, 1995·Computer Applications in the Biosciences : CABIOS·C Geourjon, G Deléage
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 14, 2000·Natural Product Reports·T M Zabriskie, M D Jackson
Dec 26, 2001·Bioinformatics·S KumarM Nei
Jun 28, 2005·Nucleic Acids Research·E QuevillonR Lopez
Jul 18, 2006·Nucleic Acids Research·Timothy L BaileyWilfred W Li
Sep 1, 2006·Cell Biochemistry and Biophysics·Hengyu XuPaul F Cook
Jan 26, 2008·Bioinformatics·Mario StankeDavid Haussler
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Feb 4, 2010·BMC Genomics·Sandrine GrossetêteOlivier Lespinet
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jason E StajichPatricia J Pukkila

❮ Previous
Next ❯

Citations

Dec 6, 2016·International Journal of Molecular Sciences·Yuan-Ping LuBao-Gui Xie
May 2, 2020·Applied Microbiology and Biotechnology·Taju WuShaojie Li
Jul 6, 2021·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Jing GuoBo Tao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.