Homocysteine-induced changes in vascular reactivity of guinea-pig pulmonary arteries: role of the oxidative stress and poly (ADP-ribose) polymerase activation

Pulmonary Pharmacology & Therapeutics
Arda TasatargilEdibe Karasu

Abstract

This study was aimed to examine the effects of homocysteine (Hcy) on vascular responsiveness of guinea-pig isolated pulmonary arteries and to investigate possible underlying mechanisms. In order to evaluate vascular reactivity, isometric tension studies were performed in response to potassium chloride (KCl), phenylephrine (Phe), acetylcholine (ACh), and sodium nitroprusside (SNP). Incubation of pulmonary artery rings with Hcy (10(-3)M, 180min) resulted in significant inhibition of response to ACh (an endothelium-dependent vasodilator)(E(max): 55.3+/-6.7 vs. 13.1+/-2.0(*), P<0.05) while SNP (an endothelium-independent vasodilator)-induced relaxation was not changed significantly. Furthermore, Hcy enhanced KCl- and Phe-induced contraction of pulmonary artery rings (E(max): 1568+/-81 vs. 2101+/-145(*)mg for KCl and 1081+/-101 vs. 1544+/-117(*)mg for Phe, P<0.05). Pulmonary artery ring contractions induced by stepwise addition to Ca(2+) to high KCl solution with no Ca(2+) were also significantly augmented by Hcy incubation (E(max): 1750+/-121 vs. 2295+/-134(*)mg, P<0.05). To investigate mechanisms of Hcy action, additional sets of experiments involving rings incubation with Hcy alone or with addition of Tiron (an intracellular supe...Continue Reading

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Citations

Aug 18, 2009·Respiratory Research·Elisabet FerrerJoan Albert Barberà
Sep 8, 2007·Antioxidants & Redox Signaling·Sanjana Dayal, Steven R Lentz
Jun 7, 2008·Digestive Diseases and Sciences·Edibe KarasuArda Tasatargil
Apr 23, 2010·Biological & Pharmaceutical Bulletin·Li-Hong QiuBi-Qi Zhang
Oct 16, 2012·Pediatrics International : Official Journal of the Japan Pediatric Society·Hale UsluerAyşe Sevim Gokalp
Jan 27, 2018·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Shalini Jamwal, Saurabh Sharma
Sep 14, 2017·Cardiovascular Therapeutics·Tawar QaradakhiAnthony Zulli
Jul 18, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Romina María UrangaGabriela Alejandra Salvador
Aug 12, 2014·Cell Biochemistry and Function·Ezgi Turunc BayrakdarAyfer Yalcin
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Dec 21, 2006·Molecular and Cellular Biochemistry·Joemerson Osório RosadoDiego Bonatto

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