Homocysteine induces hypophosphorylation of intermediate filaments and reorganization of actin cytoskeleton in C6 glioma cells.

Cellular and Molecular Neurobiology
Samanta Oliveira LoureiroRegina Pessoa-Pureur

Abstract

In this study, we investigated the actions of high homocysteine (Hcy) levels (100 and 500 microM) on the cytoskeleton of C6 glioma cells. Results showed that the predominant cytoskeletal response was massive formation of actin-containing filopodia at the cell surface that could be related with Cdc42 activation and increased vinculin immunocontent. In cells treated with 100 microM Hcy, folic acid, trolox, and ascorbic acid, totally prevented filopodia formation, while filopodia induced by 500 microM Hcy were prevented by ascorbic acid and attenuated by folic acid and trolox. Moreover, competitive NMDA ionotropic antagonist DL-AP5 totally prevented the formation of filopodia in both 100 and 500 microM Hcy treated cells, while the metabotropic non-selective group I/II antagonist MCPG prevented the effect of 100 microM Hcy but only slightly attenuated the effect induced by of 500 microM Hcy on actin cytoskeleton. The competitive non-NMDA ionotropic antagonist CNQX was not able to prevent the effects of Hcy on the reorganization of actin cytoskeleton in the two concentrations used. Also, Hcy-induced hypophosphorylation of vimentin and glial fibrillary acidic protein (GFAP) and this effect was prevented by DL-AP5, MCPG, and CNQX. In ...Continue Reading

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Citations

Nov 13, 2012·Experimental Cell Research·Samanta Oliveira LoureiroAngela T S Wyse
Sep 15, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Samanta Oliveira LoureiroRegina Pessoa-Pureur
Sep 1, 2015·Biochimica Et Biophysica Acta·Gurumayum Suraj SharmaLaishram Rajendrakumar Singh
Jul 20, 2012·Biochemistry. Biokhimii︠a︡·A A Boldyrev
Jun 26, 2017·Molecular Neurobiology·Paula Pierozan, Regina Pessoa-Pureur
Apr 1, 2021·Neurotoxicity Research·Angela T S WyseAndré Quincozes-Santos

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