Homocysteine stimulates phosphorylation of NADPH oxidase p47phox and p67phox subunits in monocytes via protein kinase Cbeta activation.

The Biochemical Journal
Yaw L SiowKarmin O

Abstract

Hyperhomocysteinaemia is an independent risk factor for cardiovascular diseases due to atherosclerosis. The development of atherosclerosis involves reactive oxygen species-induced oxidative stress in vascular cells. Our previous study [Wang and O (2001) Biochem. J. 357, 233-240] demonstrated that Hcy (homocysteine) treatment caused a significant elevation of intracellular superoxide anion, leading to increased expression of chemokine receptor in monocytes. NADPH oxidase is primarily responsible for superoxide anion production in monocytes. In the present study, we investigated the molecular mechanism of Hcy-induced superoxide anion production in monocytes. Hcy treatment (20-100 microM) caused an activation of NADPH oxidase and an increase in the superoxide anion level in monocytes (THP-1, a human monocytic cell line). Transfection of cells with p47phox siRNA (small interfering RNA) abolished Hcy-induced superoxide anion production, indicating the involvement of NADPH oxidase. Hcy treatment resulted in phosphorylation and subsequently membrane translocation of p47phox and p67phox subunits leading to NADPH oxidase activation. Pretreatment of cells with PKC (protein kinase C) inhibitors Ro-32-0432 (bisindolylmaleimide XI hydrochlo...Continue Reading

References

Apr 25, 1991·The New England Journal of Medicine·R ClarkeI Graham
Sep 12, 1974·The New England Journal of Medicine·L A HarkerR Ross
Nov 1, 1966·Analytical Biochemistry·J A Uerre, C H Miller
Feb 1, 1997·Current Opinion in Lipidology·P B Duell, M R Malinow
Jul 4, 1997·The Journal of Biological Chemistry·G R UpchurchJ Loscalzo
Jul 24, 1997·The New England Journal of Medicine·O NygårdS E Vollset
Mar 24, 1998·Annual Review of Medicine·H RefsumS E Vollset
Apr 16, 1998·The New England Journal of Medicine·G N Welch, J Loscalzo
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·G WangK O
Jan 15, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Norbert WeissJoseph Loscalzo
Mar 27, 2002·Circulation·Dan SorescuKathy K Griendling
May 23, 2002·Circulation Research·Randall S FreyAsrar B Malik
Jul 31, 2002·Journal of Clinical Pathology·L Van HeerebeekD Roos
Dec 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Sanjana DayalSteven R Lentz
Dec 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Natalia KalininaAlex Bobik
May 7, 2003·Cell·Yvonne GroempingKatrin Rittinger
Oct 4, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Martha K Cathcart
Oct 23, 2003·The Biochemical Journal·Seng-Lai Tan, Peter J Parker
Jan 28, 2004·Circulation·Jaikirshan J KhatriZorina S Galis
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erik A BeyMartha K Cathcart
Jan 13, 2005·American Journal of Physiology. Endocrinology and Metabolism·Connie W H WooKarmin O

❮ Previous
Next ❯

Citations

May 27, 2010·Archivum Immunologiae Et Therapiae Experimentalis·Vadim V Sumbayev, Sally A Nicholas
Oct 1, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Cheng-Gang ZouKe-Qin Zhang
Dec 8, 2011·Cell Biochemistry and Biophysics·S V Vijaya LakshmiVijay Kumar Kutala
Sep 8, 2007·Antioxidants & Redox Signaling·Louisa Papatheodorou, Norbert Weiss
Sep 8, 2007·Antioxidants & Redox Signaling·Edith LubosDiane E Handy
Mar 5, 2009·Antioxidants & Redox Signaling·Gregor Muller, Henning Morawietz
Apr 11, 2009·Antioxidants & Redox Signaling·Gary M BokochDavide Gianni
Feb 8, 2011·Antioxidants & Redox Signaling·Si JinPin-Lan Li
May 19, 2011·Biochemistry. Biokhimii︠a︡·E A BryushkovaA A Boldyrev
Apr 9, 2008·Journal of Leukocyte Biology·Kazuhiro OmoriThomas E Van Dyke
May 28, 2010·Neuropsychiatric Disease and Treatment·Steven F Werder
Oct 31, 2012·International Journal of Molecular Sciences·Daniela Cosentino-GomesJosé Roberto Meyer-Fernandes
Dec 11, 2007·Canadian Journal of Physiology and Pharmacology·Vathsala E R EdirimanneKarmin O
Mar 20, 2009·Canadian Journal of Physiology and Pharmacology·Fang WangZhengyuan Xia
Apr 16, 2010·Canadian Journal of Physiology and Pharmacology·Lindsei K SarnaKarmin O
Feb 9, 2012·Canadian Journal of Physiology and Pharmacology·Lindsei K SarnaKarmin O
May 29, 2007·Archives of Biochemistry and Biophysics·Samuele De Minicis, David A Brenner
Jan 31, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ching-Hsia HungKun-Ling Tsai
Jan 10, 2009·Neurobiology of Aging·Cheng-Gang ZouKe-Qin Zhang
Nov 12, 2010·Acta Biochimica Et Biophysica Sinica·Tong ZouJiefu Yang
Mar 13, 2017·Free Radical Biology & Medicine·Qian XuKathy K Griendling
Dec 23, 2014·Journal of Lipid Research·Bharat JaishyE Dale Abel
Jul 21, 2017·Bulletin of Experimental Biology and Medicine·T L KrasnikovaM V Sidorova
Jan 11, 2018·Antioxidants & Redox Signaling·Xu CaoJin-Song Bian
Mar 24, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Nima M GharaviJudith A Berliner
Oct 23, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Gábor CsányiPatrick J Pagano
Nov 8, 2006·Diabetes/metabolism Research and Reviews·Steven PattersonNeville H McClenaghan
Mar 13, 2010·Journal of Cardiovascular Pharmacology·Chen-Jing WangYuan-Jian Li
Aug 7, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Samuele De MinicisDavid A Brenner
Jun 20, 2015·Indian Journal of Clinical Biochemistry : IJCB·R DhananjayanVijay Kumar Kutala
Jan 14, 2020·Chinese Medical Journal·Yue-Miao ZhangHong Zhang
May 11, 2011·Molecular Carcinogenesis·Harish ShankaranThomas J Weber
Nov 20, 2012·Molecular and Cellular Biochemistry·Sana Venkata Vijaya LakshmiVijay Kumar Kutala
Dec 10, 2019·Journal of Immunology Research·Caitlin V LewisBarbara K Kemp-Harper
Aug 7, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nan WuKarmin O
Oct 31, 2009·European Journal of Immunology·Sabrina M Richards, Edward A Clark

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.