Homogeneity of cell envelope protein subtypes, lipopolysaccharide serotypes, and biotypes among Haemophilus influenzae type b from patients with meningitis in The Netherlands

The Journal of Infectious Diseases
L van AlphenH C Zanen

Abstract

Eighty strains of Haemophilus influenzae type b were randomly selected from 531 strains collected between 1975 and 1982 from patients with meningitis in The Netherlands. Subtyping by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that 67 of 80 isolates had identical major outer membrane protein patterns (subtype 1). Among the 13 other isolates four different polyacrylamide gel electrophoresis patterns were observed, two of which closely resembled subtype 1. Lipopolysaccharides were characterized immunologically by immunoprecipitation (Ouchterlony technique) and the gel-immuno-radio-assay. Four serotypes were found among the 80 selected strains, leaving one strain not typable. Seventy-four strains (93%) belonged to the same lipopolysaccharide serotype; 77 (97%) of 80 of the strains belonged to biotype I. Sixty strains (75%) had identical major outer membrane protein patterns (subtype 1), lipopolysaccharide serotypes (serotype 1), and biotypes (I).

Citations

Apr 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L van AlphenL Geelen
Dec 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N I Leaves, J Z Jordens
Feb 21, 1987·Lancet·K GroeneveldR J van Ketel
Aug 1, 1987·Epidemiology and Infection·A J PatersonT H Pennington
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J M MusserR K Selander
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W HoodE R Moxon
Apr 1, 1991·Archives of Disease in Childhood·A K TakalaJ Eskola
Apr 1, 1990·Microbial Pathogenesis·L van AlphenM van Ham
Jul 18, 2003·Journal of Medical Microbiology·R KaurA Chakraborti
Mar 1, 1989·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·J M BernsteinD P Dickinson
Jun 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·P Z Hønberg
Feb 7, 1998·Infection and Immunity·F GelukL van Alphen
Feb 1, 1992·Journal of Clinical Microbiology·H A BijlmerJ Dankert

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