PMID: 7016175Apr 14, 1981Paper

Homogeneity of myosin subfragments by equilibrium centrifugation

Biochemistry
S S MargossianS Lowey

Abstract

A number of enzymes are currently in use for obtaining proteolytic subfragments of rabbit skeletal muscle myosin. Subfragment-1 can be obtained by papain digestion of polymeric myosin in the presence (Mg-S1) or absence (EDTA-S1) of divalent cations [Margossian, S.S., Lowey, S., & Barshop, B. (1975) Nature (London) 258, 163-166]. Subfragment-1 prepared by chymotrypsin is readily fractionated according to its alkali light-chain content into S1(A1) and S1(A2) [Weeds, A.G., & Taylor, R.S. (1975) Nature (London) 257, 54-56]. Digestion of soluble myosin by trypsin or chymotrypsin leads to heavy meromyosin (HMM) and light meromyosin (LMM). Many of these subfragments show extensive cleavages in the heavy- and/or light-chain region by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In view of the widespread use of proteolytic subfragments in kinetics and structural studies, it was of interest to establish the extent of heterogeneity of these preparations under nondenaturing conditions by equilibrium centrifugation. Analysis of the fringe displacements by the computer programs of Roark & Yphantis [Roark, D.E., & Yphantis, D.A. (1969) Ann. N.Y. Acad. Sci. 164, 245-278] showed that for three initial loading concentrations, the m...Continue Reading

References

Dec 12, 1978·Biochemistry·S S Margossian, S Lowey
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Citations

May 8, 1990·Biochimica Et Biophysica Acta·S S MargossianH S Slayter
Jun 1, 1986·Molecular and Cellular Endocrinology·P B Mahajan, K E Ebner
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J C HearingA J Levine
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I Rayment, D A Winkelmann
Aug 1, 1989·The Journal of Cell Biology·L L Frado, R Craig
Dec 2, 1985·European Journal of Biochemistry·M Mossakowska, H Strzelecka-Gołaszewska
Nov 15, 1988·European Journal of Biochemistry·M J Redowicz, H Strzelecka-Gołaszewska
Feb 1, 1994·Current Opinion in Cell Biology·M D RozyckiU Lindberg
Aug 5, 1985·Journal of Molecular Biology·R A CrowtherR Craig
Aug 15, 1984·Journal of Molecular Biology·P Knight, J Trinick
Jun 30, 2010·PloS One·Alicia M EvangelistaWilliam H Guilford
Sep 3, 2011·PloS One·Simone KöhlerAndreas R Bausch
Jan 23, 1999·Biophysical Journal·E A BobkovaE Reisler
May 7, 2005·Journal of Molecular Biology·Stephan SchmitzClaudia Veigel
May 5, 1998·Journal of Molecular Biology·M SamsóM P Koonce
Sep 29, 2007·Journal of Molecular Biology·M J BloeminkM A Geeves
Apr 21, 2010·Biochemical and Biophysical Research Communications·Girish C MelkaniSanford I Bernstein
Jul 18, 2006·Journal of Theoretical Biology·Enrico Grazi, Cristina Di Bona
May 25, 1982·Biochemistry·J E Morel, M Garrigos
Jun 15, 1982·Biochemical and Biophysical Research Communications·J A Trotter
Feb 1, 1993·Journal of Muscle Research and Cell Motility·S S MargossianH S Slayter
Feb 14, 1992·Archives of Biochemistry and Biophysics·S I O'DonoghueC G dos Remedios

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