Homogeneous electrochemical strategy for human telomerase activity assay at single-cell level based on T7 exonuclease-aided target recycling amplification

Analytical Chemistry
Xiaojuan LiuFeng Li

Abstract

As an important biomarker for early cancer diagnostics and a valuable therapeutic target, telomerase has attracted extensive attention concerning its detection and monitoring. Herein, a homogeneous electrochemical strategy based on T7 exonuclease-aided target recycling amplification is proposed for a simple, rapid, and highly sensitive assay of human telomerase activity from crude cancer cell extracts. In this strategy, a 5' methylene blue (MB)-labeled hairpin (HP) probe is designed, which can hybridize with the telomerase reaction products to initiate the subsequent digestion by T7 exonuclease, and a large amount of MB-labeled mononucleotides are released to result in the significantly amplified electrochemical signal. By taking advantage of the high amplification efficiency of T7-aided target recycling, the present assay enables the detection of telomerase activity at the single-cell level, which is superior or comparable to that of the reported literature. Furthermore, the assay was carried out in a homogeneous solution without complex modification or immobilization procedures, which has the merits of simplicity, rapid response, and improved recognition efficiency compared with heterogeneous biosensors. With the ability of f...Continue Reading

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Citations

Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Apr 4, 2017·ACS Applied Materials & Interfaces·Lei GeFeng Li
May 30, 2017·Chemical Science·Li-Juan WangChun-Yang Zhang
Dec 4, 2015·The Analyst·Kristy S McKeatingJean-Francois Masson
Mar 10, 2016·Chemical Society Reviews·Hong-Min MengXiao-Bing Zhang
Aug 3, 2018·Chemical Communications : Chem Comm·Chen-Chen LiChun-Yang Zhang
Feb 26, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fei MaChun-Yang Zhang
Jul 7, 2020·The Analyst·Hyo Yong KimHyun Gyu Park
Feb 22, 2018·Nanoscale·Scott G HarrounAlexis Vallée-Bélisle

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