Homogeneous fluorescent immunoassay

Science
J BriggsD F Nicoli

Abstract

A fluorescent immunoassay based on the correlation of fluctuations in particle number measures the amount of tagged species bound to micrometer-sized beads and is insensitive to background fluorescence. Without separation steps, a competitive assay can resolve I nanogram of gentamicin per milliliter from a total sample volume of only 10 microliters.

References

Oct 17, 1980·Science·R J Elin

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Citations

Mar 22, 2001·Electrophoresis·Y MaE S Yeung
Sep 1, 1982·Applied Biochemistry and Biotechnology·R C Boguslaski, T M Li
Jul 16, 1985·Journal of Immunological Methods·J BriggsM J Becker
May 1, 1983·Journal of Biochemical and Biophysical Methods·H Geerts
Oct 18, 2001·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·H BazinG Mathis
Jul 27, 2001·Protein Science : a Publication of the Protein Society·A C LagerkvistR Rigler
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·N G WalterM Eigen
Mar 15, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·E S Yeung
Jul 26, 2002·Analytical Biochemistry·Sergey Y TetinEdmund D Matayoshi
Jul 25, 2000·Analytical Chemistry·M R ShortreedE S Yeung
Dec 26, 2001·Chemical Reviews·W P AmbroseR A Keller

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