Homogeneous insulin and C-Peptide sensors for rapid assessment of insulin and C-peptide secretion by the islets.

Diabetes
Ewa HeydukTomasz Heyduk

Abstract

Glucose-stimulated islet insulin or C-peptide secretion experiments are a fundamental tool for studying and assessing islet function. The goal of this work was to develop Ab-based fluorescent homogenous sensors that would allow rapid, inexpensive, near-instantaneous determinations of insulin and C-peptide levels in biological samples. Our approach was to use molecular pincer design (Heyduk et al., Anal Chem 2008;80:5152-5159) in which a pair of antibodies recognizing nonoverlapping epitopes of the target are modified with short fluorochrome-labeled complementary oligonucleotides and are used to generate a fluorescence energy transfer (FRET) signal in the presence of insulin or C-peptide. The sensors were capable of detecting insulin and C-peptide with high specificity and with picomolar concentration detection limits in times as short as 20 min. Insulin and C-peptide levels determined with the FRET sensors showed outstanding correlation with determinations performed under the same conditions with enzyme-linked immunosorbent assay. Most importantly, the sensors were capable of rapid and accurate determinations of insulin and C-peptide secreted from human or rodent islets, verifying their applicability for rapid assessment of isl...Continue Reading

References

Jan 1, 1995·Methods in Enzymology·P R Selvin
May 10, 2001·World Journal of Surgery·A BokerR Alejandro
Oct 22, 2003·Lancet·A M James ShapiroBernhard Hering
Apr 30, 2005·Analytical Chemistry·Ewa Heyduk, Tomasz Heyduk
Sep 29, 2006·The New England Journal of Medicine·A M James ShapiroJonathan R T Lakey
May 22, 2008·Analytical Chemistry·Ewa HeydukTomasz Heyduk
Jul 8, 2008·Lancet·Valter CasatiArmando D'Angelo
Jul 8, 2008·Lancet·Camillo RicordiUNKNOWN Clinical Islet Transplantation Consortium

❮ Previous
Next ❯

Citations

Dec 13, 2012·Chemical Reviews·Hongquan ZhangX Chris Le
Mar 23, 2012·American Journal of Physiology. Endocrinology and Metabolism·Aaron C BaldwinJohn A Corbett
Mar 29, 2013·Journal of the American Podiatric Medical Association·David G Armstrong, Joseph L Mills
Jul 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Oliver HenrichMichael E Cates
Feb 14, 2012·Journal of Pharmaceutical and Biomedical Analysis·Rongsheng E WangYie-Hwa Chang
Sep 17, 2013·Angewandte Chemie·Hongquan ZhangX Chris Le
Jul 11, 2014·Biosensors & Bioelectronics·Henna PäkkiläTero Soukka
May 31, 2019·The Analyst·Yixiao ShenZhimin Xu
Jan 22, 2021·Diabetes·Aaron L GliebermanKevin Kit Parker
Jul 27, 2021·Analytical Methods : Advancing Methods and Applications·Wesley J Eaton, Michael G Roper
Nov 5, 2020·Analytical Chemistry·Andresa B BezerraChristopher J Easley
Dec 12, 2018·ACS Sensors·Rebecca SoffeJames Geoffrey Chase

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
fluorescence resonance
FRET
ELISA

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.