Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis

Nucleic Acids Research
Hsin-Chih YehTza-Huei Wang

Abstract

This report describes a new genotyping method capable of detecting low-abundant point mutations in a homogeneous, separation-free format. The method is based on integration of oligonucleotide ligation with a semiconductor quantum dot (QD)-mediated two-color fluorescence coincidence detection scheme. Surface-functionalized QDs are used to capture fluorophore-labeled ligation products, forming QD-oligonucleotide nanoassemblies. The presence of such nanoassemblies and thereby the genotype of the sample is determined by detecting the simultaneous emissions of QDs and fluorophores that occurs whenever a single nanoassembly flows through the femtoliter measurement volume of a confocal fluorescence detection system. The ability of this method to detect single events enables analysis of target signals with a multiple-parameter (intensities and count rates of the digitized target signals) approach to enhance assay sensitivity and specificity. We demonstrate that this new method is capable of detecting zeptomoles of targets and achieve an allele discrimination selectivity factor >10(5).

References

Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F Barany
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D A NickersonU Landegren
Aug 26, 1988·Science·U LandegrenL Hood
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Eigen, R Rigler
Oct 1, 1996·Genome Research·C A HeidP M Williams
Oct 10, 1997·Analytical Chemistry·A Castro, J G Williams
Feb 3, 1998·Nature Biotechnology·S TyagiF R Kramer
Sep 25, 1998·Science·M BruchezA P Alivisatos
Mar 20, 1999·Genetic Analysis : Biomolecular Engineering·K J Livak
Mar 20, 1999·Genetic Analysis : Biomolecular Engineering·S A MarrasS Tyagi
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·B Vogelstein, K W Kinzler
Aug 26, 2000·Analytical Chemistry·J P KnemeyerM Sauer
Nov 10, 2001·Annual Review of Genomics and Human Genetics·P Y Kwok
Dec 6, 2001·Nature Reviews. Genetics·A C Syvänen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·M M Mhlanga, L Malmberg
Jul 26, 2002·Nucleic Acids Research·Brian W KirkFrancis Barany
Aug 9, 2002·Journal of the American Chemical Society·Dustin J MaxwellShuming Nie
Jan 9, 2003·Pharmacogenomics·Richard M Twyman, Sandy B Primrose
Apr 23, 2003·Analytical Chemistry·Haitao LiDavid Klenerman
Jul 3, 2003·Journal of the American Chemical Society·Kae SatoMizuo Maeda
Apr 17, 2004·Nucleic Acids Research·Kyoko TakatsuTakahiro Kanagawa
Jul 2, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kae SatoMizuo Maeda
Jul 21, 2004·Nucleic Acids Research·Jonne VaarnoAleksi E Soini
Apr 14, 2005·Journal of the American Chemical Society·Tza-Huei WangChih-Ming Ho
Jun 2, 2005·Nature Materials·Igor L MedintzHedi Mattoussi
Sep 6, 2005·Nucleic Acids Research·Guo-Hua ZhouHideki Kambara
Nov 1, 2005·Nucleic Acids Research·Jishan LiRu-Qin Yu
Dec 28, 2005·Nature Materials·Chun-Yang ZhangTza-Huei Wang

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Citations

Apr 7, 2010·Journal of the American Chemical Society·Kelvin J LiuTza-Huei Wang
Jul 11, 2012·Journal of the American Chemical Society·Hsin-Chih YehJames H Werner
Apr 8, 2009·Lab on a Chip·Christopher M Puleo, Tza-Huei Wang
Nov 14, 2007·Tissue Engineering·Christopher M PuleoTza-Huei Wang
Aug 9, 2008·International Journal of Nanomedicine·Igor L MedintzAaron R Clapp
Aug 24, 2012·Theranostics·Yi Zhang, Tza-Huei Wang
Dec 18, 2013·Analytical Chemistry·Wei Wang, Nongjian Tao
Jun 4, 2013·Advanced Drug Delivery Reviews·Katla Sai KrishnaChalla S S R Kumar
Jun 19, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshihiro Ihara, Motoko Mukae
Jun 19, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Heyou HanZonghai Sheng
Jun 1, 2010·Analytical and Bioanalytical Chemistry·Changbei Ma, Edward S Yeung
Feb 2, 2011·Chemical Communications : Chem Comm·Rohan T Ranasinghe, Tom Brown
Jul 23, 2008·The Analyst·Daniel MeservePak Kin Wong
Jul 6, 2013·Nanoscale·Judy M OblioscaHsin-Chih Yeh
Apr 1, 2009·Nature Chemistry·Pavel Anzenbacher, Manuel A Palacios
Feb 18, 2014·Advanced Healthcare Materials·Monty LiongRalph Weissleder
Jan 13, 2010·Lab on a Chip·T D RaneT H Wang
Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Noritada KajiYoshinobu Baba
Nov 25, 2017·Chemical Communications : Chem Comm·Juan HuChun-Yang Zhang
Nov 23, 2007·Chembiochem : a European Journal of Chemical Biology·Fumihiko Fujii, Masataka Kinjo
Sep 24, 2008·Angewandte Chemie·Ron GillItamar Willner
May 28, 2019·Current Molecular Medicine·Somayeh Heidari SharafdarkolaeeFatemeh Heidari Sharafdarkolaee
May 20, 2006·Clinical Chemistry·Hassan M E AzzazySteven C Kazmierczak
May 4, 2017·Journal of the American Chemical Society·Judy M OblioscaHsin-Chih Yeh

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Methods Mentioned

BETA
electrophoresis
flow
fluorescence
PCR
laser capture microdissection
Assay
genotyping
flow cytometry

Software Mentioned

LabView

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