Homogenisation and oxygen transfer rates in large agitated and sparged animal cell bioreactors: Some implications for growth and production.

Cytotechnology
Alvin W NienowN K Slater

Abstract

Because of concern for cell damage, very low agitation energy inputs have been used in industrial animal cell bioreactors, typical values being two orders of magnitude less than those found in bacterial fermentations. Aeration rates are also very small. As a result, such bioreactors might be both poorly mixed and also unable to provide the higher oxygen up-take rates demanded by more intensive operation. This paper reports experimental studies both of K( L ) a and of mixing (via pH measurements) in bioreactors up to 8 m(3) at Wellcome and of scaled down models of such reactors at Birmingham. Alongside these physical measurements, sensitivity of certain cell lines to continuously controlled dO(2) has been studied and the oxygen up-take rates measured in representative growth conditions. An analysis of characteristic times and mixing theory, together with other recent work showing that more vigorous agitation and aeration can be used especially in the presence of Pluronic F-68, indicates ways of improving their performance. pH gradients offer a special challenge.

References

Jan 1, 1994·Cytotechnology·V LeelavatcharamasM al-Rubeai

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Citations

Nov 30, 1999·Biotechnology and Bioengineering·C Langheinrich, A W Nienow
Jul 13, 2002·Biotechnology and Bioengineering·Jason J OsmanJulie Varley
Sep 6, 2001·Biotechnology and Bioengineering·J J OsmanJ Varley
Nov 13, 2008·Cytotechnology·Alvin W Nienow
Nov 13, 2008·Cytotechnology·J Varley, J Birch
Nov 13, 2008·Cytotechnology·David M Marks
Mar 13, 2009·Biotechnology and Bioengineering·Zizhuo XingSteven S Lee
Jul 26, 2006·Biotechnology and Bioengineering·Anli Ouyang, Shang-Tian Yang
Dec 17, 2005·Biotechnology and Bioengineering·F MeuwlyU von Stockar
Apr 13, 2011·Biotechnology Progress·Joseph J Bellucci, Kent H Hamaker
Mar 8, 2012·Biotechnology Journal·Carl-Fredrik Mandenius, Mats Björkman
Sep 10, 2009·Biotechnology and Bioengineering·Timothy A BarrettGary J Lye
Feb 22, 2018·Biotechnology Progress·Sen XuHao Chen
Aug 9, 2018·Bioprocess and Biosystems Engineering·Rubin JiangSen Xu
Sep 19, 2020·Engineering in Life Sciences·Katrin PaulChristoph Herwig

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