Homogenization of initial cell distribution by secondary flow of medium improves cell culture efficiency.

PloS One
Yuki FukumaKenjiro Takemura

Abstract

Homogenization of the initial cell distribution is essential for effective cell development. However, there are few previous reports on efficient cell seeding methods, even though the initial cell distribution has a large effect on cell proliferation. Dense cell regions have an inverse impact on cell development, known as contact inhibition. In this study, we developed a method to homogenize the cell seeding density using secondary flow, or Ekman transportation, induced by orbital movement of the culture dish. We developed an orbital shaker device that can stir the medium in a 35-mm culture dish by shaking the dish along a circular orbit with 2 mm of eccentricity. The distribution of cells in the culture dish can be controlled by the rotational speed of the orbital shaker, enabling dispersion of the initial cell distribution. The experimental results indicated that the cell density became most homogeneous at 61 rpm. We further evaluated the cell proliferation after homogenization of the initial cell density at 61 rpm. The results revealed 36% higher proliferation for the stirred samples compared with the non-stirred control samples. The present findings indicate that homogenization of the initial cell density by Ekman transport...Continue Reading

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Citations

Dec 1, 2020·Journal of Bioscience and Bioengineering·Takuma KuriyamaKenjiro Takemura
Feb 1, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mohammad AbbasJeffrey A Stuart
Feb 20, 2021·Ultrasonics Sonochemistry·Genichiro FujiiKenjiro Takemura
Jul 10, 2021·The Journal of the Acoustical Society of America·Taigo OyamaKenjiro Takemura

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