Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization

Journal of Proteomics
Marcel KwiatkowskiHartmut Schlüter

Abstract

Posttranslational modifications and proteolytic processing regulate almost all physiological processes. Dysregulation can potentially result in pathologic protein species causing diseases. Thus, tissue species proteomes of diseased individuals provide diagnostic information. Since the composition of tissue proteomes can rapidly change during tissue homogenization by the action of enzymes released from their compartments, disease specific protein species patterns can vanish. Recently, we described a novel, ultrafast and soft method for cold vaporization of tissue via desorption by impulsive vibrational excitation (DIVE) using a picosecond-infrared-laser (PIRL). Given that DIVE extraction may provide improved access to the original composition of protein species in tissues, we compared the proteome composition of tissue protein homogenates after DIVE homogenization with conventional homogenizations. A higher number of intact protein species was observed in DIVE homogenates. Due to the ultrafast transfer of proteins from tissues via gas phase into frozen condensates of the aerosols, intact protein species were exposed to a lesser extent to enzymatic degradation reactions compared with conventional protein extraction. In addition, ...Continue Reading

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Citations

Feb 15, 2018·Nature Chemical Biology·Ruedi AebersoldBing Zhang
Aug 8, 2019·Mass Spectrometry Reviews·Irena DapicGarry L Corthals
May 1, 2020·Metallomics : Integrated Biometal Science·M OmidiB J C Luthringer-Feyerabend
Mar 25, 2019·Future Science OA·Lorena HänelHartmut Schlüter
Mar 3, 2021·ACS Nano·Carlos Sanchez-CanoWolfgang J Parak
Nov 6, 2020·Journal of Proteome Research·Sarah A MichaudChristoph H Borchers
May 13, 2021·Clinical Oral Investigations·Yaghoup Feridouni KhamanehReinhard E Friedrich
Jun 11, 2021·PloS One·Jessica RauchAnke Osterloh

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Datasets Mentioned

BETA
PXD003434
The

Methods Mentioned

BETA
biopsy
electrophoresis
acetylation

Software Mentioned

DIVE
Andromeda
Delta2D
MaxQuant
Proteome Discoverer
LFQ
DAVID
Sequest HT

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