PMID: 9426617Jan 14, 1998Paper

Homologous and heterologous protein import into mitochondria isolated from the green alga Chlamydomonas reinhardtii

Plant Molecular Biology
G NuraniL G Franzén

Abstract

We have established a homologous system for studying mitochondrial protein import in Chlamydomonas reinhardtii, using C. reinhardtii precursor proteins and mitochondria isolated from C. reinhardtii. The precursors of the F1 alpha ATP synthase subunit and the Rieske FeS protein were imported into mitochondria with high efficiency, while the F1 beta subunit precursor was imported with much lower efficiency. The import of heterologous precursor proteins from higher plants was also less efficient. The precursor of the C. reinhardtii PsaF chloroplast protein was converted into a protease-protected form upon incubation with mitochondria. In vitro processing studies revealed that in contrast to the situation in higher plants, the processing of the precursors was catalysed by a soluble, matrix-located peptidase.

Citations

May 22, 2008·Genetics·Francisco Figueroa-MartínezDiego González-Halphen
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Charlotta RudheElzbieta Glaser
Sep 28, 2004·The Journal of Biological Chemistry·Christine SchönfeldOlaf Kruse

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