Homologous recombination occurs frequently at innate GT microsatellites in normal somatic and germ cells in vivo

BMC Genomics
Jianbo ZhengChen Luo

Abstract

In somatic cells, homologous recombination (HR) is a rare event caused by eventual DNA double-strand breaks (DSBs). In contrast, germ cells show high frequency of HR caused by programmed DSBs. Microsatellites are prone to DSBs during genome replication and, thereby, capable of promoting HR. It remains unclear whether HR occurs frequently at microsatellites both in normal somatic cells and germ cells in a similar manner. By examining the linkage pattern of multiple paternal and maternal markers flanking innate GT microsatellites, we measured HR at the GT microsatellites in various somatic cells and germ cells in a goldfish intraspecific heterozygote. During embryogenesis, the HR products accumulate gradually with the increase of the number of cell divisions. The frequency of HR at the GT microsatellites in advanced embryos, adult tissues and germ cells is surprisingly high. The type of exchanges between the homologous chromosomes is similar in normal advanced embryos and germ cells. Furthermore, a long GT microsatellite is more active than a short one in promoting HR in both somatic and germ cells. HR occurs frequently at innate GT microsatellites in normal somatic cells and germ cells in a similar manner.

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Citations

Jul 15, 2021·Gene Therapy·Jessica D GumersonTiansen Li

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Datasets Mentioned

BETA
KU870661
KU870662

Methods Mentioned

BETA
PCR

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