PMID: 9529389May 16, 1998Paper

Homology-dependent gene silencing in Paramecium

Molecular Biology of the Cell
Françoise RuizL Madeddu

Abstract

Microinjection at high copy number of plasmids containing only the coding region of a gene into the Paramecium somatic macronucleus led to a marked reduction in the expression of the corresponding endogenous gene(s). The silencing effect, which is stably maintained throughout vegetative growth, has been observed for all Paramecium genes examined so far: a single-copy gene (ND7), as well as members of multigene families (centrin genes and trichocyst matrix protein genes) in which all closely related paralogous genes appeared to be affected. This phenomenon may be related to posttranscriptional gene silencing in transgenic plants and quelling in Neurospora and allows the efficient creation of specific mutant phenotypes thus providing a potentially powerful tool to study gene function in Paramecium. For the two multigene families that encode proteins that coassemble to build up complex subcellular structures the analysis presented herein provides the first experimental evidence that the members of these gene families are not functionally redundant.

References

Jan 1, 1992·Developmental Genetics
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J A KanabrockiC Kung
Feb 1, 1990·Trends in Genetics : TIG·M Jerka-Dziadosz, J Beisson
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R GodiskaJ R Preer
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Dec 25, 1984·Journal of Molecular Biology·A AdoutteJ Beisson
Feb 1, 1995·Current Opinion in Cell Biology·J L Salisbury
Jan 1, 1995·Trends in Genetics : TIG·M A Matzke, A J Matzke
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J GaertigM A Gorovsky
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R B Flavell
May 15, 1996·European Journal of Biochemistry·L MadedduJ Beisson
Jun 1, 1997·Current Opinion in Cell Biology·A Depicker, M V Montagu
Aug 8, 1997·Cell·P M Bingham
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Cogoni, G Macino
Dec 1, 1996·Journal of Neurogenetics·W J HaynesC Kung

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Citations

Apr 12, 2008·Purinergic Signalling·Todd M Hennessey
Aug 16, 2008·Applied Biochemistry and Biotechnology·Pengda MaXingzhi Wang
May 3, 2002·Molecular and Biochemical Parasitology·Zachary N AdelmanAnthony A James
Jun 12, 2003·Molecular and Biochemical Parasitology·Mickaël Durand-DubiefPhilippe Bastin
May 30, 2001·Trends in Genetics : TIG·P DessenJ Cohen
Dec 26, 2001·Trends in Genetics : TIG·Angélique Galvani, Linda Sperling
Aug 26, 1999·Trends in Genetics : TIG·A Fire
Jun 24, 2000·Biochimie·L VayssiéJ Cohen
Apr 24, 2001·Research in Microbiology·P BastinL Sperling
Mar 15, 2001·Current Opinion in Cell Biology·R W Carthew
Feb 7, 2001·Current Opinion in Biotechnology·S W Ding
Apr 8, 2000·Current Opinion in Genetics & Development·J A BirchlerU Bhadra
Jan 16, 1999·Current Biology : CB·F RuizP Dupuis-Williams
Feb 3, 2000·Nature Cell Biology·J M Bosher, M Labouesse
May 22, 2001·Molecular Microbiology·F GraïaM Picard
Nov 30, 2005·Molecular Biology of the Cell·Thomas WassmerHelmut Plattner
Jul 31, 2003·Nucleic Acids Research·Mitch Raponi, Greg M Arndt
Dec 24, 2008·Nucleic Acids Research·Gersende LepèreEric Meyer
Aug 11, 2001·Molecular Plant-microbe Interactions : MPMI·C J MooreR M MacDiarmid
Nov 29, 2002·Eukaryotic Cell·Linda SperlingJean Cohen
Nov 29, 2002·Eukaryotic Cell·Alex HaddadAaron P Turkewitz
Feb 12, 2004·Eukaryotic Cell·F RuizF Koll
Apr 22, 2009·Molecular and Cellular Biology·Eva-Maria LadenburgerHelmut Plattner
Aug 18, 2004·Molecular and Cellular Biology·Olivier GarnierEric Meyer
Aug 2, 2011·Annual Review of Genomics and Human Genetics·Mariusz NowackiLaura F Landweber
Feb 11, 2005·BMC Biotechnology·Filippo RusconiPhilippe Bastin
May 31, 2006·PLoS Pathogens·Rivka BrachaDavid Mirelman
Jul 1, 2007·Frontiers of Medicine in China·Yewei JiYuanguo Zhou
Nov 8, 2002·Proceedings of the National Academy of Sciences of the United States of America·W John HaynesRobin R Preston
Jul 14, 2014·Nucleic Acids Research·Estienne C SwartMariusz Nowacki
Dec 29, 2000·The Journal of Eukaryotic Microbiology·J R Preer
Jan 29, 2000·The Journal of Eukaryotic Microbiology·C KungR Kissmehl

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