Homology of the 70-kilodalton antigens from Mycobacterium leprae and Mycobacterium bovis with the Mycobacterium tuberculosis 71-kilodalton antigen and with the conserved heat shock protein 70 of eucaryotes.

Infection and Immunity
R J GarsiaA Basten

Abstract

Two lambda gt11 recombinant clones, JKL2 and JKL15, each containing an insert coding for part of the highly immunogenic 70-kilodalton (kDa) protein antigen, were isolated from a Mycobacterium leprae genomic library by immunoscreening with the monoclonal antibody L7. Clone JKL2 contained the largest insert, 2.3 kilobase pairs. Nonoverlapping fragments of this insert were used as probes and showed strong hybridization to a number of Mycobacterium tuberculosis-lambda gt11 recombinants producing proteins recognized by an anti-M. tuberculosis 71-kDa monoclonal antibody, IT11. One clone from a recombinant Mycobacterium bovis library was also characterized by using L7, and the insert from this clone, B5bt, hybridized strongly to the M. leprae probes as well. The nucleotide sequence of the 1,037-base-pair coding region of the JKL2 M. leprae clone which encodes the carboxy-terminal half of the 70-kDa protein had extensive homology with genes from a number of species. In all cases, these genes, including the recently described Ag63 and Ag361 of Plasmodium falciparum, were found to be members of the heat shock protein 70 (hsp 70) family of genes. At the amino acid level, homology was maximal between amino acids 83 through 107 and 159 thro...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V MehraR A Young
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A YoungR W Davis
Mar 22, 1985·Science·D J Lipman, W R Pearson
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A E BiancoD J Kemp
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R N Husson, R A Young
Apr 1, 1988·Infection and Immunity·A J RadfordP Plackett
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Hunt, R I Morimoto
Oct 25, 1985·Nucleic Acids Research·K C Reed, D A Mann
Aug 1, 1984·The Journal of Cell Biology·D M SchlossmanJ E Rothman
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R A Young, R W Davis
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J C Bardwell, E A Craig
Oct 1, 1981·The Journal of Hygiene·S J StanleyI Sutherland

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Citations

Apr 1, 1991·European Journal of Immunology·A Wand-WürttenbergerS H Kaufmann
Jan 1, 1995·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·P B SugermanG J Seymour
Apr 15, 1996·FEMS Microbiology Letters·R Segal, E Z Ron
Apr 8, 2006·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Milan Raska, Evzen Weigl
Sep 1, 1990·Scandinavian Journal of Immunology·M HarboeP J Lachmann
Apr 1, 1991·Digestive Diseases and Sciences·D C MarkesichD Y Graham
May 1, 1994·Clinical and Experimental Immunology·S Khanolkar-YoungD N Lockwood
Nov 30, 1994·Experientia·G Del Giudice
Jun 1, 1993·Japanese Journal of Cancer Research : Gann·T TorigoeK Kikuchi
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·B J PrakkenW van Eden
Jul 26, 2019·BMC Pediatrics·Sobana MuthuveluAnand Mohan
Aug 1, 1996·Molecular Aspects of Medicine·M J Colston
Feb 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J EichlerI Silman
May 1, 1992·Journal of Bacteriology·M WetzsteinW Schumann
Jan 9, 1999·Clinical Microbiology Reviews·U Zügel, S H Kaufmann
Sep 1, 1990·Research in Microbiology·J E Clark-CurtissR E Esser
Jul 1, 1994·Infection and Immunity·D P HarrisJ Ivanyi
Sep 1, 1989·Research in Immunology·P Dubois
Dec 1, 1992·Microbiological Reviews·H G Wiker, M Harboe

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