Homophilic interactions between cadherin fragments at the single molecule level: an AFM study

Langmuir : the ACS Journal of Surfaces and Colloids
O du RoureP Silberzan

Abstract

We report measurements of the adhesion forces between single E-cadherin fragments anchored on solid surfaces. These fragments consist of the two outermost extracellular domains of the protein. The specificity of the measured rupture forces was demonstrated by Ca2+ exchange experiments. Two series of experiments were performed using two linkers of different rigidity and length. We find that the pull-off force is distributed with a maximum value independent of the linker and logarithmically dependent on the velocity of separation of the two surfaces. Our dynamical results are compatible with previous flow chamber experiments performed with the same fragments and can be compared from a different perspective with previously reported AFM experiments on the full-length extracellular domain of the VE-cadherin. Interestingly, using a rigid linker, we have been able for the first time to evidence the deformation of the cadherin molecule under mechanical stress, a piece of information not accessible with more classical grafting strategies.

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Citations

Dec 1, 2007·Journal of Biological Physics·Fredy Zypman
Aug 12, 2009·Cell Biochemistry and Biophysics·Georgi Z GenchevHui Lu
Jun 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lea-Laetitia PontaniJasna Brujic
Nov 26, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Leda RaptisHélène Feracci
Jan 21, 2016·Biophysical Journal·Lea-Laetitia PontaniJasna Brujic
Aug 4, 2011·Journal of Molecular Recognition : JMR·Małgorzata LekkaPiotr Laidler
Oct 25, 2017·Scientific Reports·Mareike DiedingDario Anselmetti
Mar 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Connor J ThompsonDaniel K Schwartz
May 22, 2008·Journal of Cell Science·Jonne HeleniusDaniel J Muller
Jul 25, 2019·The Journal of Physical Chemistry Letters·Connor J ThompsonDaniel K Schwartz

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