Homotypic cell contact enhances insulin but not glucagon secretion

Biochemical and Biophysical Research Communications
Helen BreretonP M Jones

Abstract

Intra-islet interactions influence beta-cell function, and disruption of islet architecture results in a reduction in glucose-induced insulin secretion, whereas re-aggregation improves secretory responsiveness. Our studies on MIN6 cells have shown that by configuring beta-cells as three-dimensional islet-like structures there is a marked improvement in glucose-induced insulin secretion compared to that of their monolayer equivalents. In the present study, we have used the mouse glucagon-secreting alphaTC1 cell line to see whether homotypic interactions are important in the regulation of glucagon secretion from alpha-cells. We found no significant difference in the secretory responses of alphaTC1 cells maintained as monolayers or as cell clusters. We also found that different cell adhesion molecules are involved in cell interactions between alpha- and beta-cells; MIN6 cells express ECAD, whereas alphaTC1 cells express NCAM. ECAD is necessary for cell cluster formation by MIN6 cells but not by alphaTC1 cells, whereas NCAM is not needed for the formation of cell clusters in either cell line.

References

Nov 1, 1975·The Journal of Clinical Endocrinology and Metabolism·L OrciR Unger
Sep 1, 1988·Journal of Molecular Endocrinology·T BjaalandS L Howell
Sep 1, 1985·Endocrinology·D G PipeleersM Van de Winkel
Aug 15, 1973·Experientia·L OrciA E Renold
Jun 14, 2002·Molecular and Cellular Endocrinology·A C Hauge-EvansP M Jones
Oct 14, 2004·American Journal of Physiology. Endocrinology and Metabolism·Melanie J LutherPeter M Jones
Mar 15, 2006·Biochemical and Biophysical Research Communications·Melanie J LutherPeter M Jones

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Citations

Jan 15, 2009·Cell and Tissue Research·Ihsane MarhfourYves Guiot
May 11, 2012·Biomedical Microdevices·Daniel Gallego-PerezKeith J Gooch
Mar 12, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Adam D MendelsohnTejal A Desai
Aug 30, 2008·Tissue Engineering. Part a·Laney M WeberKristi S Anseth
Aug 29, 2013·Journal of Biomedical Nanotechnology·Vuk Uskoković
Jul 1, 2011·Stem Cells International·Catriona KellyNeville H McClenaghan
Sep 14, 2007·Archives of Physiology and Biochemistry·Peter M JonesShanta J Persaud
Aug 21, 2012·Acta Biomaterialia·Adam D MendelsohnTejal A Desai
Aug 24, 2012·Journal of Tissue Engineering and Regenerative Medicine·Hyunjung BaekHeechung Kwon
Apr 14, 2016·Experimental Cell Research·Alastair D GreenPeter R Flatt
Mar 18, 2015·Journal of Cellular and Molecular Medicine·Janneke HilderinkAart van Apeldoorn
Mar 5, 2014·Organogenesis·Nobuhiko Kojima
Jul 20, 2010·Biochemical and Biophysical Research Communications·Catriona KellyNeville H McClenaghan
Mar 17, 2018·The International Journal of Artificial Organs·Katarzyna SkrzypekDimitrios Stamatialis
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Gareth J RogersPaul E Squires
Jun 24, 2018·Bioengineering·Cláudia C MirandaJoaquim M S Cabral
May 25, 2021·Experimental Cell Research·Devi Prasad Vijayashankar, Tushar Vaidya

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