Homozygous deletion of MKK4 in ovarian serous carcinoma.

Cancer Biology & Therapy
Kentaro NakayamaTian-Li Wang

Abstract

Analysis of deleted chromosomal regions in tumors has historically led to the identification of tumor suppressor genes. In this study, we used digital karyotyping, a genome-wide, high-resolution technology, to search for chromosomal deletions in ovarian serous carcinoma, the most lethal gynecological malignancy in women. Five purified ovarian serous carcinomas were analyzed by digital karyotyping and small interstitial deletions at chromosome 17p were identified in two tumor samples. Aligning these two deletions identified an overlapping region that spanned 2.4 Mb which harbored a candidate tumor suppressor gene, mitogen-activated protein kinase kinase-4 (MKK4). Dual-color FISH analysis confirmed homozygous deletion of the MKK4 locus in both samples and RT-PCR demonstrated that both carcinomas lacked MKK4 transcript expression. Loss of heterozygosity of 17p occurred in 24 (86%) of 28 high-grade serous carcinomas including both cases with homozygous MKK4 deletion. Additionally, downregulation of MKK4 expression was found in 96 (75%) of 128 ovarian serous carcinomas as compared to benign ovarian tissues. These findings suggest that homozygous deletion or reduced expression of MKK4 may contribute to the development of ovarian sero...Continue Reading

Citations

Aug 4, 2006·Molecular Diagnosis & Therapy·Ritu SalaniTian-Li Wang
Apr 14, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shamima YeasminKohji Miyazaki
Jan 18, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Ie-Ming ShihTian-Li Wang
Oct 25, 2007·Nature Reviews. Cancer·Julio A Aguirre-Ghiso
Apr 8, 2010·Science Translational Medicine·Rebecca J LearyVictor E Velculescu
Apr 17, 2013·Journal of Hematology & Oncology·Akintunde AkinleyeDelong Liu
Oct 31, 2012·International Journal of Molecular Sciences·Kentaro NakayamaKohji Miyazaki
Feb 20, 2016·ELife·Vidhu SharmaUNKNOWN Reproducibility Project Cancer Biology
Feb 22, 2011·European Journal of Cell Biology·Wiebke HaeusgenVicki Waetzig
Jul 18, 2009·International Journal of Cancer. Journal International Du Cancer·Yisheng WeiJianping Wang
Mar 24, 2010·International Journal of Cancer. Journal International Du Cancer·Shamima YeasminKohji Miyazaki
Feb 4, 2014·Biochimica Et Biophysica Acta·Wiebke HaeusgenVicki Waetzig
Mar 30, 2017·Journal of Medical Genetics·Seungchul LeeHyunju Lee
Mar 14, 2007·International Journal of Cancer. Journal International Du Cancer·Kentaro NakayamaTian-Li Wang
May 15, 2007·Oncogene·A J Whitmarsh, R J Davis
May 15, 2007·Oncogene·A S DhillonW Kolch
Sep 17, 2010·Nature Reviews. Cancer·Neal G Copeland, Nancy A Jenkins
Jul 1, 2015·Oncotarget·Goldie Y L LuiDes R Richardson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.