Homozygous deletion of the p16INK4a and the p15INK4b tumour suppressor genes in a subset of human sporadic cutaneous malignant melanoma

The British Journal of Dermatology
S N WagnerM Goos

Abstract

Chromosome 9p21 is frequently deleted in malignant melanoma, and one familial malignant melanoma gene has been linked to 9p21-22. Recently, the cyclin D-dependent kinase inhibitors (CDKIs) p16INK4a and p15INK4b have been localized within chromosome 9p21, and the presence of p16INK4a point mutations has been demonstrated in familial melanoma and melanoma cell lines in vitro. To analyse the role of these CDKIs in sporadic human cutaneous non-metastatic malignant melanoma, we examined 36 primary tumour specimens representing different stages of melanoma progression and their corresponding normal skin samples for the three mechanisms of CDKI inactivation described so far. Homozygous codeletion of the p16INK4a and the p15INK4b gene was detected by Southern blot analysis in two tumour samples. By direct sequencing of polymerase chain reaction (PCR)-amplified microdissected genomic DNA; no somatic or germline p16INK4a point mutations or small deletions were detected in the remaining 34 tumour samples; one individual exhibited the previously described germline codon 148 (Ala-->Thr) polymorphism. In these tumour specimens, comparative semiquantitative reverse PCR analysis of p16INK4a transcript levels revealed no evidence for repression...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1995·The Journal of Investigative Dermatology·S N WagnerM Goos
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·A OkamotoD H Beach
Jul 15, 1994·Science·P CairnsD Sidransky
Jul 21, 1994·Nature·C H SpruckP A Jones
Dec 5, 1995·Biochemical and Biophysical Research Communications·M TenanG Finocchiaro
Sep 1, 1996·The Journal of Investigative Dermatology·E HealyJ L Rees
Oct 16, 1996·Journal of the National Cancer Institute·W G YarbroughE T Liu
Aug 1, 1996·Melanoma Research·W A RobinsonP Hersey

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Citations

Jun 18, 2005·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·José Luis Soto MartínezMiguel Angel López-Nevot
Feb 15, 2002·Lancet·Majid ShahbaziIan V Hutchinson
Oct 2, 2001·Pharmacology & Therapeutics·M LeszczynieckaP B Fisher
Aug 14, 2002·The British Journal of Dermatology·J E Osborne, P E Hutchinson
Apr 7, 2000·Carcinogenesis·S KazianisR S Nairn
May 16, 2012·Journal of the American Academy of Dermatology·Katherine A WardMaria K Hordinsky
May 8, 1999·The Journal of Investigative Dermatology·K PerisP Wolf
Feb 2, 2008·Brain and Language·Diane L KendallStephen E Nadeau
Mar 21, 2009·The British Journal of Dermatology·P KanellouD A Spandidos
May 20, 2003·The British Journal of Dermatology·Z SaridakiD A Spandidos
Mar 10, 2001·Experimental Cell Research·J W Rocco, D Sidransky
Oct 8, 2005·Seminars in Oncology·Marcella MacalusoAntonio Giordano
May 11, 2012·The Biochemical Journal·Young-Woong KimSeong-Jin Kim
Apr 15, 2008·Cancer Genetics and Cytogenetics·Zsuzsa RákosyMargit Balázs
Apr 18, 2012·PloS One·Jan HönnemannHans-Peter Elsässer
Dec 18, 1998·Science·P SunD Beach
Jul 10, 2008·British Journal of Cancer·F LesueurHélène Zattara
Sep 26, 2000·American Journal of Physiology. Cell Physiology·J N RaoJ Y Wang
Dec 1, 1998·Genes & Development·L ChinR A DePinho
Apr 25, 2000·Journal of the American Academy of Dermatology·M Piepkorn

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