Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide

Frontiers in Microbiology
Yunfei WuTatsuhiko Kadowaki

Abstract

The negative effects of honey bee parasitic mites and deformed wing virus (DWV) on honey bee and colony health have been well characterized. However, the relationship between DWV and mites, particularly viral replication inside the mites, remains unclear. Furthermore, the physiological outcomes of honey bee immune responses stimulated by DWV and the mite to the host (honey bee) and perhaps the pathogen/parasite (DWV/mite) are not yet understood. To answer these questions, we studied the tripartite interactions between the honey bee, Tropilaelaps mercedesae, and DWV as the model. T. mercedesae functioned as a vector for DWV without supporting active viral replication. Thus, DWV negligibly affected mite fitness. Mite infestation induced mRNA expression of antimicrobial peptides (AMPs), Defensin-1 and Hymenoptaecin, which correlated with DWV copy number in honey bee pupae and mite feeding, respectively. Feeding T. mercedesae with fruit fly S2 cells heterologously expressing honey bee Hymenoptaecin significantly downregulated mite Vitellogenin expression, indicating that the honey bee AMP manipulates mite reproduction upon feeding on bee. Our results provide insights into the mechanism of DWV transmission by the honey bee parasitic...Continue Reading

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Citations

Mar 2, 2021·Frontiers in Microbiology·Yunfei WuTatsuhiko Kadowaki

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Datasets Mentioned

BETA
PRJNA608093
OQR67440.1

Methods Mentioned

BETA
PCR
Protein
protein assay
RNA-seq

Software Mentioned

Bioconductor edgeR
MEGA7
HTSeq
Hisat2
R
MUSCLE
count
build indexer
SAMtools
Image Lab TM

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